Protective effect of bergamot (Citrus medica L. var. sarcodactylis Swingle) extract on acute alcoholic gastric ulcer

IF 4.8 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience Pub Date : 2025-06-10 DOI:10.1016/j.fbio.2025.107012

Li Xiaolin , Wu Zhen , Luo Liyong , Zeng Liang , Wang Yuanhui , Wang Yongde , Luo Wei

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Abstract

This study investigates the protective effects of bergamot (Citrus medica L. var. sarcodactylis Swingle) extract on acute alcoholic gastric ulcers in mice model. The extract was analyzed for its chemical composition, total phenol and flavonoid contents, and antioxidant activities using DPPH and ABTS assays. Acute gastric ulcers were induced in mice with anhydrous ethanol, and the protective effects of the extract were evaluated by assessing gastric tissue morphology, serum superoxide dismutase (SOD), malondialdehyde (MDA), and inflammatory markers (TNF-α, IL-6, and IL-1β). LC-MS analysis revealed 674 components in the extract, including flavonoids, saponins, and other bioactive compounds. Quantification analysis of individual phenolic compounds such as gallic acid, ferulic acid, p-coumaric acid, hesperidin, rutin, neohesperidin, naringin, eriocitrin, limettin, narirutin, oxypeucedanin, and narcissoside showed a total amount of 1451 mg/kg. The total phenol content was 4.74 ± 0.24 % DW (dry weight), and the total flavonoid content was 0.64 ± 0.04 % DW. The extract showed good antioxidant activity, with DPPH and ABTS IC₅₀ values of 287.65 ± 5.38 μg mL⁻¹ and 326.31 ± 10.89 μg mL⁻¹ respectively. Treatment with bergamot extract significantly decreased the gastric ulcer index, increased the ulcer inhibition rate, reduced MDA levels, and increased SOD activity, improving gastric mucosal injury. Furthermore, inflammatory markers TNF-α, IL-6, and IL-1β were significantly reduced (p < 0.01). These findings suggest that bergamot extract may offer effective protection against alcohol-induced gastric injury.

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佛手柑提取物对急性酒精性胃溃疡的保护作用

本研究探讨佛手柑提取物对急性酒精性胃溃疡小鼠模型的保护作用。采用DPPH和ABTS法对提取物的化学成分、总酚和类黄酮含量以及抗氧化活性进行了分析。采用无水乙醇诱导小鼠急性胃溃疡，通过观察胃组织形态、血清超氧化物歧化酶（SOD）、丙二醛（MDA）和炎症标志物（TNF-α、IL-6和IL-1β）来评价其保护作用。LC-MS分析发现提取物中含有674种成分，包括类黄酮、皂苷和其他生物活性化合物。对没食子酸、阿魏酸、对香豆酸、橙皮苷、芦丁、新橙皮苷、柚皮苷、芥子皮苷、限定素、柚皮苷、氧去核苷、水仙苷等酚类化合物的定量分析显示，总含量为1451 mg/kg。总酚含量为4.74±0.24% DW（干重），总黄酮含量为0.64±0.04% DW。提取物具有良好的抗氧化活性，DPPH和ABTS IC50值分别为287.65±5.38 μ mL - 1和326.31±10.89 μ mL - 1。佛手柑提取物可显著降低胃溃疡指数，提高溃疡抑制率，降低MDA水平，提高SOD活性，改善胃粘膜损伤。此外，炎症标志物TNF-α、IL-6和IL-1β显著降低(p <；0.01)。这些发现表明佛手柑提取物可能对酒精引起的胃损伤有有效的保护作用。

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来源期刊

Food Bioscience Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

6.40

自引率

5.80%

发文量

671

审稿时长

27 days

期刊介绍： Food Bioscience is a peer-reviewed journal that aims to provide a forum for recent developments in the field of bio-related food research. The journal focuses on both fundamental and applied research worldwide, with special attention to ethnic and cultural aspects of food bioresearch.