A Multifunctional Tetrahedral DNA Nanostructure for the Simultaneous Sensitive Detection and Imaging of miRNA and Zinc Ions in Living Cells

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2025-06-21 DOI:10.1021/acs.analchem.5c01674

Qinghong Jiang, Jie Zhou, Yuwei Sha, Mengshi Jiang, Yaqin Chai, Ruo Yuan

{"title":"A Multifunctional Tetrahedral DNA Nanostructure for the Simultaneous Sensitive Detection and Imaging of miRNA and Zinc Ions in Living Cells","authors":"Qinghong Jiang, Jie Zhou, Yuwei Sha, Mengshi Jiang, Yaqin Chai, Ruo Yuan","doi":"10.1021/acs.analchem.5c01674","DOIUrl":null,"url":null,"abstract":"In this study, a multifunctional tetrahedral DNA nanostructure (MTDN) was constructed by assembling different functional modules for the simultaneous sensitive detection and imaging of targets miRNA-21 and Zn2+ in living cells related to liver cancer. Impressively, compared with the traditional detection strategy for miRNA-21 and Zn2+ with low sensitivity, this strategy employs autocatalytic hairpin assembly (ACHA) to rapidly self-assemble into linear tetrahedral DNA nanostructures (LTDNS) by activating the functional module on MTDN to produce two strong fluorescent signals for sensitive detection of miRNA-21 and Zn2+ with detection limits of 0.79 pM and 33.46 nM, respectively, which were much lower than the reported literature. More importantly, LTDNS with a spatial confinement effect and low diffusion coefficient could achieve accurate localization imaging of targets to significantly enhance imaging clarity. The strategy provides an innovative method for simultaneous sensitive detection and accurate imaging of multiple biomarkers and has broad application prospects in early disease diagnosis.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"15 1","pages":""},"PeriodicalIF":6.7000,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1021/acs.analchem.5c01674","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

In this study, a multifunctional tetrahedral DNA nanostructure (MTDN) was constructed by assembling different functional modules for the simultaneous sensitive detection and imaging of targets miRNA-21 and Zn²⁺ in living cells related to liver cancer. Impressively, compared with the traditional detection strategy for miRNA-21 and Zn²⁺ with low sensitivity, this strategy employs autocatalytic hairpin assembly (ACHA) to rapidly self-assemble into linear tetrahedral DNA nanostructures (LTDNS) by activating the functional module on MTDN to produce two strong fluorescent signals for sensitive detection of miRNA-21 and Zn²⁺ with detection limits of 0.79 pM and 33.46 nM, respectively, which were much lower than the reported literature. More importantly, LTDNS with a spatial confinement effect and low diffusion coefficient could achieve accurate localization imaging of targets to significantly enhance imaging clarity. The strategy provides an innovative method for simultaneous sensitive detection and accurate imaging of multiple biomarkers and has broad application prospects in early disease diagnosis.

Abstract Image

查看原文本刊更多论文

一种多功能四面体DNA纳米结构用于活细胞中miRNA和锌离子的同时灵敏检测和成像

本研究通过组装不同的功能模块，构建了多功能四面体DNA纳米结构（MTDN），用于肝癌相关活细胞靶点miRNA-21和Zn2+的同时灵敏检测和成像。令人印象深刻的是，与传统的低灵敏度的miRNA-21和Zn2+检测策略相比，该策略通过激活MTDN上的功能模块，利用自催化发夹组装（ACHA）快速自组装成线性四边形DNA纳米结构（LTDNS），产生两个强荧光信号，对miRNA-21和Zn2+进行灵敏检测，检测限分别为0.79 pM和33.46 nM，远低于文献报道。更重要的是，LTDNS具有空间约束效应和低扩散系数，可以实现目标的精确定位成像，显著提高成像清晰度。该策略为多种生物标志物的同时灵敏检测和准确成像提供了一种创新方法，在疾病早期诊断中具有广阔的应用前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.