A Novel Electrochemiluminescence Sensor for Sensitive Detection of Estriol Based on "Three-in-One" Nanocomposite.

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2025-06-20 DOI:10.1021/acs.analchem.5c01270

Shuyue Xing,Xuejing Liu,Dawei Fan,Zhongfeng Gao,Hongmin Ma,Huan Wang,Dan Wu,Qin Wei

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引用次数: 0

Abstract

In this study, chemically polymerized luminol-hydrogen-bonded organic framework (CpL-HOF) is used as the efficient luminophore and POMs@MOFs as the quencher to construct the electrochemiluminescence (ECL) sensor for the ultrasensitive detection of estriol (E3). CpL-HOF is prepared through coordination self-assembly using chemically polymerized luminol (CpL) and three active ligands. The nanoconfinement effect of CpL-HOF exhibits enhanced stability and ECL efficiency compared with the monomer. Mo-based polyoxometalates (Mo POMs) as guests are encapsulated inside a metal-organic framework (Cu-MOF) to synthesize POMs@MOFs nanocomposites with "three-in-one" functions. The distinctive host-guest interaction enhances the activity of superoxide dismutase-like (SOD-like) and catalase-like (CAT-like) enzymes, effectively eliminating reactive oxygen species (ROS). Furthermore, POMs@MOFs exhibit electron-rich redox properties, which can further eliminate ROS by valence switching. Therefore, POMs@MOFs can effectively quench the ECL signal of the CpL-HOF and improve the sensitivity of the sensor. Under optimal conditions, the sensor has a wide linear range (100 fg/mL to 200 ng/mL) and a low limit of detection (92.84 fg/mL). The sensor can be applied to the analysis of real samples, with satisfactory results. The ECL mechanism based on the synergistic interaction of the host-guest provides a theoretical foundation for constructing novel ECL sensing platforms.

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基于“三合一”纳米复合材料的新型电化学发光传感器对雌三醇的灵敏检测。

本研究以化学聚合的鲁米醇-氢键有机骨架（cl - hof）为高效发光团，POMs@MOFs为猝灭剂，构建了用于超灵敏检测雌三醇（E3）的电化学发光（ECL）传感器。用化学聚合发光氨（CpL）和三种活性配体通过配位自组装制备了CpL- hof。与单体相比，CpL-HOF的纳米约束效应表现出更高的稳定性和ECL效率。将钼基多金属氧酸盐（Mo POMs）作为客体封装在金属有机框架（Cu-MOF）中，合成具有“三合一”功能的POMs@MOFs纳米复合材料。独特的主-客体相互作用增强了超氧化物歧化酶样（SOD-like）和过氧化氢酶样（CAT-like）酶的活性，有效地消除活性氧（ROS）。此外，POMs@MOFs表现出富电子的氧化还原特性，可以通过价交换进一步消除ROS。因此，POMs@MOFs可以有效地淬灭cpll - hof的ECL信号，提高传感器的灵敏度。在最佳条件下，该传感器具有宽线性范围（100 fg/mL至200 ng/mL）和低检测限（92.84 fg/mL）。该传感器可用于实际样品的分析，结果令人满意。基于主客体协同作用的ECL机制为构建新型ECL传感平台提供了理论基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.