Comparative Metabolic Profiling of Wild and In Vitro Cultivated Cecropia angustifolia Roots Fraction Reveals Conservation of Pentacyclic Triterpene Acid Biosynthesis.

IF 3 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Phytochemical Analysis Pub Date : 2025-06-18 DOI:10.1002/pca.70001

Luis M Díaz-Sánchez, Martha L Chacón-Patiño, Cristian Blanco-Tirado, Marianny Y Combariza, Guillermo Montoya

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引用次数: 0

Abstract

Introduction: Cecropia angustifolia roots are a traditional source of pentacyclic triterpene acids (PTAs), bioactive compounds with promising therapeutic potential. In vitro cultivation offers a sustainable alternative to metabolite production while minimizing the impact on wild populations.

Objective: To compare the metabolic profiles of wild and in vitro cultivated C. angustifolia roots using ultrahigh-resolution mass spectrometry.

Methods: Root fractions from wild and in vitro cultivated materials were analyzed using negative-ion electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI(-)-FT-ICR-MS) at 21 Tesla.

Results: Molecular analysis revealed diverse PTA species in both wild and in vitro root fractions. While in vitro cultivation produced a more streamlined metabolic profile, PTA content remained significantly enriched.

Conclusion: In vitro cultivation of C. angustifolia roots represents a viable strategy for sustainably producing pharmacologically relevant PTAs, supporting both conservation and the biotechnological use of natural products.

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野生和体外培养天蚕根提取物代谢谱的比较分析揭示了五环三萜酸生物合成的保守性。

摘要：柞木根是一种传统的五环三萜酸（pta）的来源，是一种具有良好治疗潜力的生物活性化合物。体外培养提供了一种可持续的代谢物生产替代方案，同时最大限度地减少对野生种群的影响。目的：采用超高分辨率质谱法比较野生和体外培养的金针叶根的代谢谱。方法：采用21特斯拉负离子电喷雾电离傅里叶变换离子回旋共振质谱（ESI(-)- ft - icr - ms）对野生和离体栽培材料的根组分进行分析。结果：分子分析表明，野生和离体根中PTA种类多样。虽然体外培养产生了更精简的代谢谱，但PTA含量仍然显著丰富。结论：金针叶根的体外培养是一种可持续生产药理学相关PTAs的可行策略，支持天然产物的保护和生物技术利用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Phytochemical Analysis 生物-分析化学

CiteScore

6.00

自引率

6.10%

发文量

审稿时长

1.7 months

期刊介绍： Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.