The hydrogenation metabolism process of rosmarinic acid by microbial enzymes in chickens.

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-06-18 DOI:10.1002/jsfa.70000

Xuedong Ding, Jing Wang, Weiyun Zhu

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引用次数: 0

Abstract

Background: The gut microbiota plays a critical role in the metabolism of rosmarinic acid (RA) through converting RA to caffeic acid (CA), danshensu (DSS), and m-coumaric acid. However, the gut environment and microbiota may have complicated metabolic converting RA processes. This study aimed to investigate the metabolism of RA in gastrointestinal tract of chickens through liquid chromatography-tandem mass spectrometry (LC-MS/MS) and metagenomic analysis.

Results: Through in vivo and in vitro studies, RA was found to be hydrogenated into dihydrorosmarinic acid, then hydrolyzed into DSS and dihydrocaffeic acid (DHCA). DSS and DHCA could be further converted to 3-hydroxyphenylpropionic acid. But RA remained stable in the stomach, duodenum, jejunum, and ileum, as well as in the cecum of antibiotic-treated chickens. This indicated that the degradation of RA was mainly mediated by cecal microbiota. Furthermore, the metagenomic analysis of cecal microbiota revealed that reductases and hydrolases from Clostridium spp., Alistipes spp., and other microbiota were involved in these processes. NADH:flavin oxidoreductase and 3-oxoacyl-[acyl-carrier-protein] reductase participated in the hydrogenation reaction of RA, and BaiCD involved in dehydroxylation reaction of RA.

Conclusion: The hydrogenation process by microbial enzymes was an important metabolic pathway of RA. These hydrogenation products contribute to improving the biological function of RA. © 2025 Society of Chemical Industry.

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鸡体内微生物酶对迷迭香酸的加氢代谢过程。

背景：肠道菌群通过将迷迭香酸（RA）转化为咖啡酸（CA）、丹参素（DSS）和间香豆酸，在迷迭香酸（RA）的代谢中起着关键作用。然而，肠道环境和微生物群可能具有复杂的代谢转化RA过程。本研究旨在通过液相色谱-串联质谱（LC-MS/MS）和宏基因组分析研究RA在鸡胃肠道中的代谢。结果：通过体内和体外研究，发现RA氢化成二氢丙氨酸，再水解成DSS和二氢咖啡酸（DHCA）。DSS和DHCA可进一步转化为3-羟基苯基丙酸。但类风湿性关节炎在使用抗生素的鸡的胃、十二指肠、空肠、回肠以及盲肠中保持稳定。这表明RA的降解主要是由盲肠菌群介导的。此外，盲肠微生物群的宏基因组分析表明，来自Clostridium spp., Alistipes spp.和其他微生物群的还原酶和水解酶参与了这些过程。NADH：黄素氧化还原酶和3-氧酰-[酰基-载体蛋白]还原酶参与RA的加氢反应，BaiCD参与RA的去羟基化反应。结论：微生物酶加氢过程是RA的重要代谢途径。这些氢化产物有助于改善RA的生物学功能。©2025化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.