Improving the thermal tolerance of biocontrol spores, Akanthomyces lecanii, by encapsulation.

IF 2.2 4区生物学 Q3 MICROBIOLOGY

Fems Microbiology Letters Pub Date : 2025-06-16 DOI:10.1093/femsle/fnaf062

Paul W Baker, Ephraim Nuwamanya, Adam Charlton

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Abstract

Akanthomyces lecanii is an entomopathogenic fungus, and spores of this fungus could be incorporated into films generated using cast film extrusion for biocontrol applications. However, the extrusion process involves high temperature processing (150°C) although this only lasts for a few minutes. The elevated temperature destroys spores, thereby eliminating functionality, unless the spores are protected from this heat. Initial experiments revealed that the heat tolerance of free A. lecanii spores to be 60°C. The spores were therefore encapsulated into beads prepared using a combination of Gelrite, cellulose and Cel-fine at different concentrations. The beads were freeze-dried and then immersed in hot glycerol for 2 minutes at a selected temperature within the range of 50-100°C. The results indicated that some combinations of encapsulating agents resulted in the spores retaining viability (plate counting) after heat treatment at 100°C. Beads stored at room temperature for one week showed a reduction in the upper temperature tolerance. This study revealed that the temperature tolerance of A. lecanii spores could be improved by 40°C by encapsulation in freeze-dried beads containing 2% Gelrite (purified gellum gum), 0.4% cellulose and 0.4% Cel-fine.

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用包封法提高生防孢子的耐热性。

lecanomyces lecanii是一种昆虫病原真菌，该真菌的孢子可以加入到用铸膜挤出法生产的薄膜中，用于生物防治。然而，挤压过程涉及高温处理（150°C），尽管这只持续几分钟。升高的温度会破坏孢子，从而破坏其功能，除非孢子能免受高温的影响。初步实验表明，游离芽孢杆菌孢子的耐热性可达60℃。因此，孢子被包裹在由凝胶、纤维素和不同浓度的细胞细粒混合而成的小珠中。将微球冷冻干燥，然后在50-100℃范围内的选定温度下在热甘油中浸泡2分钟。结果表明，某些包封剂组合可使孢子在100℃热处理后保持活力（平板计数）。在室温下储存一周的珠子显示出高温耐受性的降低。本研究表明，在含有2%明胶、0.4%纤维素和0.4%细胞细粒的冻干珠粒中包埋，可将蜡芽孢孢子的耐温性提高40℃。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fems Microbiology Letters 生物-微生物学

CiteScore

4.30

自引率

0.00%

发文量

112

审稿时长

1.9 months

期刊介绍： FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered. 2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020) Ranking: 98/135 (Microbiology) The journal is divided into eight Sections: Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies) Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens) Biotechnology and Synthetic Biology Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses) Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies) Virology (viruses infecting any organism, including Bacteria and Archaea) Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature) Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology) If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.