SlWRKY30, a positive regulator of resistance to Meloidogyne incognita in Solanum lycopersicum

IF 3.9 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2025-06-01 DOI:10.1016/j.scienta.2025.114224

Yule Dang , Zhize Wang , Weidan Nie , Yinxia Chen , Yushan Li , Yue Ma , Yu Song , Xiang Li , Chong Du

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引用次数: 0

Abstract

WRKY transcription factors play important roles in plant biological stress responses. Through RNA-seq, SlWRKY30 was shown to be differentially expressed in Mi-1- and Mi-3-resistant tomato lines after inoculation with Meloidogyne incognita. Bioinformatics analysis and expression profiling of roots from different lines revealed that this gene may serve as a key node for disease resistance regulation. This study employed VIGS to investigate whether the silencing of SlWRKY30 could affect the susceptibility of ‘AC’ (mi-1/mi-1) susceptible line and ‘18,060′ (Mi-1/Mi-1) resistant line to M. incognita. Subcellular localization analysis indicated that the protein encoded by this gene is located in the nucleus. Its overexpression in tomato significantly increased disease resistance, and the tomato plant’s resistance level increased from highly susceptible to resistant. Indirectly, these findings suggested that the regulation of resistance by SlWRKY30 might occur downstream of the Mi-mediated process or that the signal pathway involved by SlWRKY30 was different from that mediated by Mi. Combined with the measurement of growth (plant height, stem diameter, leaf area, etc.) and physiological (soluble sugars and proteins, root activity, etc.) indicators, these findings further clarify the role of SlWRKY30 as a positive regulatory factor in tomato defense against M. incognita. A preliminary exploration of metabolic signals revealed that SlWRKY30 may stimulate different types of signaling, such as salicylic acid, jasmonic acid and brassinosteroid signaling, etc., and may synergistically regulate reactive oxygen species accumulation through influencing scavenging enzyme activity, hindering the formation of feeding sites and ultimately leading to a reduction in root galls growth. To our knowledge, SlWRKY30 exhibits significant potential for enhancing tomato resistance to root-knot nematodes and holds great value in breeding applications.

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番茄茄（Solanum lyopersicum）对嗜甜蛾（meloidogyina incognita）抗性的正调控因子SlWRKY30

WRKY转录因子在植物生物胁迫反应中发挥着重要作用。通过RNA-seq分析，发现SlWRKY30在接种了米-1和米-3抗性番茄品系后存在差异表达。不同品种根系的生物信息学分析和表达谱分析表明，该基因可能是抗病调控的关键节点。本研究采用VIGS技术研究了SlWRKY30基因沉默是否会影响‘AC’（mi-1/mi-1）易感品系和‘18060’（mi-1/mi-1）抗性品系对M. incognita的易感性。亚细胞定位分析表明，该基因编码的蛋白位于细胞核内。其在番茄中的过表达显著提高了番茄的抗病性，植株的抗病性水平由高感提高到抗性。这些发现间接表明，SlWRKY30对抗性的调控可能发生在Mi介导过程的下游，或者SlWRKY30参与的信号通路与Mi介导的信号通路不同。结合生长（株高、茎粗、叶面积等）和生理（可溶性糖和蛋白、根系活性等）指标的测量，这些研究结果进一步阐明了SlWRKY30作为一个积极的调节因子在番茄对黑僵菌的防御中的作用。对代谢信号的初步探索发现，SlWRKY30可能刺激水杨酸、茉莉酸和油菜素内酯等不同类型的信号，并可能通过影响清除酶活性协同调节活性氧的积累，阻碍取食位点的形成，最终导致根瘿生长减少。据我们所知，SlWRKY30在提高番茄对根结线虫的抗性方面具有显著的潜力，在育种上具有重要的应用价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.