Advances in Cellobiose Dehydrogenases: Molecular Modifications and High-Throughput Screening for Biotechnological Applications

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-06-11 DOI:10.1021/acs.jafc.5c00980

Pengfei Li, Yong Feng, Zhengfen Wu, Muhammad Saeed, Chaoyang Hu, Daochen Zhu and Huayou Chen*,

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Abstract

Cellobiose dehydrogenase (CDH), a key biomass oxidoreductase, holds significant potential for applications in fields such as straw feed, biofuels, and bioelectrochemical sensors. However, the limitations of natural CDH in terms of catalytic efficiency, substrate specificity, and stability have hindered its widespread industrial application. This review provides a comprehensive overview of the structure, classification, and catalytic mechanism of CDH. Special attention has been given to progress in molecular engineering approaches such as rational design and high-throughput screening. Moreover, this review discusses future directions, such as the interplay between CDH and cellulases, self-mutagenesis-driven evolution, and innovative high-throughput screening strategies. The aim is to offer valuable insights for further studies and potential applications of CDH, ultimately advancing industrialization processes in fields such as straw feed and bioelectricity.

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纤维素二糖脱氢酶的研究进展：分子修饰及生物技术应用的高通量筛选

纤维素二糖脱氢酶（CDH）是一种重要的生物质氧化还原酶，在秸秆饲料、生物燃料和生物电化学传感器等领域具有重要的应用潜力。然而，天然CDH在催化效率、底物特异性和稳定性方面的局限性阻碍了其广泛的工业应用。本文综述了CDH的结构、分类和催化机理。特别关注分子工程方法的进展，如合理设计和高通量筛选。此外，本文还讨论了CDH与纤维素酶的相互作用、自我诱变驱动的进化以及创新的高通量筛选策略等未来研究方向。目的是为CDH的进一步研究和潜在应用提供有价值的见解，最终推动秸秆饲料和生物电等领域的工业化进程。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.