Label-free three-dimensional cellular detection and analysis using holographic tomography and Raman tweezers spectroscopy

IF 4.6 2区物理与天体物理 Q1 OPTICS

Optics and Laser Technology Pub Date : 2025-06-11 DOI:10.1016/j.optlastec.2025.113346

Chung-Hsuan Huang , Han-Yen Tu , Chau-Jern Cheng

引用次数: 0

Abstract

This paper presents an innovative multimodal detection system that integrates holographic tomography with Raman tweezers spectroscopy for label-free cellular detection, providing three-dimensional (3D) cell morphology and biochemical composition analysis. Holographic tomography (HT) provides detailed 3D cell morphological images and spatial coordinates, while Raman tweezers spectroscopy (RTS) is employed to analyze biochemical characteristics within specific region of interest (ROI) based on a novel coordinate linking technique for effective connection between HT space and RTS space. The system achieves precise probing and detection with a positioning accuracy of 120 nm and an axial accuracy of 400 nm. Experimental results demonstrate its effectiveness in detecting and analyzing the 3D internal structures and the biochemical compositions of the human retinal pigment epithelial (ARPE-19) cells.

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使用全息断层扫描和拉曼镊子光谱的无标签三维细胞检测和分析

本文提出了一种创新的多模态检测系统，该系统集成了全息断层扫描和拉曼镊子光谱，用于无标签细胞检测，提供三维（3D）细胞形态和生化成分分析。全息断层扫描（HT）提供详细的三维细胞形态图像和空间坐标，而拉曼镊子光谱（RTS）基于一种新颖的坐标链接技术，在HT空间和RTS空间之间有效连接，用于分析特定感兴趣区域（ROI）内的生化特征。该系统实现了精确的探测和检测，定位精度为120纳米，轴向精度为400纳米。实验结果表明，该方法可用于检测和分析人眼视网膜色素上皮细胞（ARPE-19）的三维内部结构和生化成分。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Optics and Laser Technology 物理-光学

CiteScore

8.50

自引率

10.00%

发文量

1060

审稿时长

3.4 months

期刊介绍： Optics & Laser Technology aims to provide a vehicle for the publication of a broad range of high quality research and review papers in those fields of scientific and engineering research appertaining to the development and application of the technology of optics and lasers. Papers describing original work in these areas are submitted to rigorous refereeing prior to acceptance for publication. The scope of Optics & Laser Technology encompasses, but is not restricted to, the following areas: •development in all types of lasers •developments in optoelectronic devices and photonics •developments in new photonics and optical concepts •developments in conventional optics, optical instruments and components •techniques of optical metrology, including interferometry and optical fibre sensors •LIDAR and other non-contact optical measurement techniques, including optical methods in heat and fluid flow •applications of lasers to materials processing, optical NDT display (including holography) and optical communication •research and development in the field of laser safety including studies of hazards resulting from the applications of lasers (laser safety, hazards of laser fume) •developments in optical computing and optical information processing •developments in new optical materials •developments in new optical characterization methods and techniques •developments in quantum optics •developments in light assisted micro and nanofabrication methods and techniques •developments in nanophotonics and biophotonics •developments in imaging processing and systems