Synergistic effects of compound plant extracts and Lactobacillus plantarum on osteogenesis: prebiotic potential and mechanistic insights from metabolomics and molecular docking.

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-06-07 DOI:10.1002/jsfa.14412

Mingjie Jia, Jiayuan Luo, Yihong Bao, Xu Zhang, Yangyang Chai, Fangfei Li, Shilong Jiang, Qinggang Xie

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引用次数: 0

Abstract

Background: Osteoporosis is a major public health challenge and innovative dietary strategies are needed to support bone health.

Results: This study aimed to develop a functional food by promoting osteogenesis through the synergistic effects of compound plant extracts (CPE) and Lactobacillus plantarum. First, it was determined that CPE could promote the growth, metabolic activity, biofilm formation, calcium enrichment, and antioxidant properties of L. plantarum, improving the bioavailability of calcium. Cell experiments showed that the combined application of CPE and L. plantarum (CPEL) significantly promoted the proliferation, differentiation, and extracellular matrix mineralization of MC3T3-E1 cells. Mechanistically, CPEL activated the Wnt/β-catenin and BMP2/Smad signaling pathways, which are essential for bone formation. The multi-omics integration of metabolomics and molecular docking revealed key metabolic changes, particularly in amino acid and carbohydrate metabolic pathways, contributing to enhanced osteogenic activity. Molecular docking also clarified the strong binding interactions between differential metabolites (leucine, salicylic acid, and fumaric acid) and osteogenic targets, providing new insights into plant component-microbe synergy in functional food development.

Conclusion: The combined application of CPE and L. plantarum could promote bone formation through multiple targets and pathways. These findings highlight the potential of plant extract-probiotic synergy in developing functional foods for bone health, offering a promising dietary strategy to prevent osteoporosis. © 2025 Society of Chemical Industry.

查看原文本刊更多论文

复合植物提取物和植物乳杆菌对成骨的协同作用：代谢组学和分子对接的益生元潜力和机制见解。

背景：骨质疏松症是一个重大的公共卫生挑战，需要创新的饮食策略来支持骨骼健康。结果：本研究旨在通过复合植物提取物（CPE）与植物乳杆菌的协同作用，开发一种促进骨生成的功能性食品。首先，CPE可以促进植物乳杆菌的生长、代谢活性、生物膜形成、钙富集和抗氧化性能，提高钙的生物利用度。细胞实验表明，CPE和L. plantarum （CPEL）联合应用可显著促进MC3T3-E1细胞的增殖、分化和细胞外基质矿化。从机制上讲，CPEL激活了Wnt/β-catenin和BMP2/Smad信号通路，这是骨形成所必需的。代谢组学的多组学整合和分子对接揭示了关键的代谢变化，特别是氨基酸和碳水化合物代谢途径，有助于增强成骨活性。分子对接还阐明了差异代谢物（亮氨酸、水杨酸和富马酸）与成骨靶点之间的强结合相互作用，为功能性食品开发中的植物组分-微生物协同作用提供了新的见解。结论：CPE与植物乳杆菌联合应用可通过多靶点、多途径促进骨形成。这些发现强调了植物提取物-益生菌协同作用在开发骨骼健康功能食品中的潜力，为预防骨质疏松症提供了一种有希望的饮食策略。©2025化学工业协会。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.