Multi-omics profiling of Amorphophallus muelleri seeds: Insights into germination and ABA regulation

IF 4.2 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2025-06-01 DOI:10.1016/j.scienta.2025.114217

Yongteng Zhao , Min Yang , Qingwei Wang , Lifang Li, Penghua Gao, Ying Qi, Jian-Wei Guo, Huanyu Wei, Jiani Liu, Jianrong Zhao, Feiyan Huang, Lei Yu

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引用次数: 0

Abstract

Amorphophallus muelleri, a naturally occurring variant of konjac, holds considerable ecological, economic, and medicinal significance due to its high konjac glucomannan (KGM) content. While the importance of seed germination as a complex physiological process is widely recognized, the regulatory network governing konjac seed germination remains largely unexplored. To better understand the fundamental molecular processes, we conducted a simultaneous examination of the transcriptome and non-targeted metabolome profiles of A. muelleri seeds at seven stages of germination, including dry seed, seed protrusion, seed germination; seedling coating outcrop, seedling cotyledons formation, seedling leaf with brown, and seedling leaf with green. Transcriptome and metabolome analyses revealed a reprogramming of seed metabolism during germination, marked by changes in phytohormones, flavonoids, starch, and sucrose. The levels of abscisic acid (ABA) were found to decline, particularly post-germination. A CYP707A gene (DN5757_c0_g1) linked to ABA catabolism was associated with reduced ABA levels during seed germination. The upregulation of mRNAs encoding cinnamic acid 4-hydroxylase (C4H) correlated with an increase in p-coumaric acid levels. Full activation of photosynthesis facilitated seedling growth at the post-germination stage. Using weighted gene co-expression network analysis (WGCNA), two modules linked to plant hormone biosynthesis, phenylpropanoid metabolism, and flavonoid biosynthesis were identified. Significantly, further results suggest that ABA may inhibit the germination process by involving gene expression. Using an exploratory multiomics dataset, our findings provide a comprehensive understanding of the germination process of A. muelleri seeds, elucidating the temporal-specific expression patterns of key candidate genes and metabolites, as well as shedding light on the role of ABA in the germination of konjac seeds.

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魔芋种子的多组学分析：对萌发和ABA调控的见解

魔芋葡甘露聚糖（KGM）含量高，是一种天然的魔芋变种，具有重要的生态、经济和药用价值。虽然种子萌发作为一个复杂的生理过程的重要性已被广泛认识，但魔芋种子萌发的调控网络仍在很大程度上未被探索。为了更好地了解其基本的分子过程，我们同时检测了毛蕊草种子在萌发的7个阶段的转录组和非靶向代谢组谱，包括干燥种子、种子突出、种子萌发和萌发；苗包衣露头，苗子叶形成，苗叶呈褐色，苗叶呈绿色。转录组和代谢组分析揭示了种子在萌发过程中代谢的重编程，以植物激素、类黄酮、淀粉和蔗糖的变化为标志。发现脱落酸（ABA）水平下降，特别是在萌发后。一个与ABA分解代谢相关的CYP707A基因（DN5757_c0_g1）与种子萌发过程中ABA水平的降低有关。编码肉桂酸4-羟化酶（C4H）的mrna的上调与对香豆酸水平的增加相关。充分激活光合作用有利于幼苗萌发后的生长。利用加权基因共表达网络分析（WGCNA），鉴定了与植物激素生物合成、苯丙素代谢和类黄酮生物合成相关的两个模块。值得注意的是，进一步的结果表明，ABA可能通过参与基因表达来抑制发芽过程。利用探索性多组学数据，我们的研究结果提供了对魔芋种子萌发过程的全面了解，阐明了关键候选基因和代谢物的时间特异性表达模式，并揭示了ABA在魔芋种子萌发中的作用。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.