Design and optimization of NPK fertilization for ginger (Zingiber officinale) seed rhizome production from tissue-cultured plantlets in soilless culture

IF 3.9 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2025-06-01 DOI:10.1016/j.scienta.2025.114208

Hui Wang , Fardous Mohammad Safiul Azam , Xiaojun Xiao , Farhana Tasnim , Pranta Das , Xie Zhou , Hongyuan Gan

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Abstract

This abstract presents a standardized NPK fertilization protocol for producing ginger seed rhizomes from pre-basic seed rhizomes derived from tissue-cultured plantlets. We examined the effects of NPK fertilization in a soilless medium on the cultivation of ‘pre-basic’ to ‘basic' seed rhizomes, providing disease-free planting materials via an L₉(3⁴) orthogonal experimental design. During the early growth phase, N2P1K3 (90 mg l^-1 N + 20 mg l^-1 P + 180 mg l^-1 K) has significantly enhanced photosynthesis and leaf growth, resulting the highest net photosynthetic rate, stomatal conductance, intercellular CO₂ concentration, and transpiration rate. K and P significantly regulates the photosynthetic traits, which were associated with increased leaf counts and taller plants, indicators of overall plant vigor. In the late growth stage, N3K2 (300 mg l^-1 N + 400 mg l^-1 K) supported vigorous rhizome development, yielding bulky rhizomes with numerous fingers. Nitrogen significantly influenced tiller number, finger number and overall plant biomass. These findings highlight the importance of a balanced NPK regimen in enhancing the quantity and quality of seed rhizomes. Our technical approach could facilitate the large-scale production of healthy basic seed rhizomes in ginger cultivation.

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无土组织培养姜种子根茎生产氮磷钾施肥设计与优化

本文介绍了一种标准化的氮磷钾施肥方案，用于从组织培养植株的预碱性种子根茎中产生姜种子根茎。我们通过L9（34）正交试验设计，研究了在无土培养基中施用氮磷钾对“预碱性”到“碱性”种子根茎培养的影响，提供了无病种植材料。在生长早期，N2P1K3 （90 mg l-1 N + 20 mg l-1 P + 180 mg l-1 K）显著促进了光合作用和叶片生长，导致净光合速率、气孔导度、细胞间CO2浓度和蒸腾速率最高。钾和磷显著调节了叶片数增加和植株长高等光合性状。在生长后期，N3K2 （300 mg l-1 N + 400 mg l-1 K）支持根茎的旺盛发育，产生粗大的根茎，根茎有许多指状。氮肥对分蘖数、指数和植物总生物量影响显著。这些发现强调了平衡氮磷钾方案在提高种子根茎数量和质量方面的重要性。本技术方法可促进生姜种植中健康基本种子根茎的规模化生产。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.