Miniaturization of the QuEChERS method in fruits and vegetables without cryogenic milling: approach to greener analysis of pesticide residues.

IF 2.2
Yuki Yamasaki, Ivan Aloisi, Hans Mol
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Abstract

The present study explored the feasibility of miniaturizing the QuEChERS method for pesticide residue analysis in three types of fruit and vegetables comminuted under ambient conditions. Laboratory samples of > 1-2 kg of spinach, orange, and red grape containing 12 sprayed pesticides which cover broad range of physicochemical properties and up to 6 incurred pesticides were comminuted using either a universal food cutter/mixer (1-step comminution) or a combination of the cutter/mixer followed by further homogenization using an UltraTurrax (2-step comminution). Analytical test portions of different weights (15, 2, and 0.5 g) were subsequently collected, respectively. The weighed test portions were extracted with proportionally scaled-down amounts of acetonitrile and salts, and analyzed by LC-MS/MS. The results showed no problematic variability in analytical results, even for test portions as small as 0.5 g for sprayed and incurred pesticides in all commodities tested. The optimal comminution approach depended on the commodities: while 1-step comminution sufficed in most cases, certain commodities required 2-step comminution to provide sufficient homogeneity for miniaturization. The QuEChERS method can be miniaturized to a 0.5 g test portion for the commodities tested without cryogenic milling, enabling laboratories to conduct a more environmentally friendly analysis of pesticide residues.

微型QuEChERS方法在水果和蔬菜中没有低温碾磨:农药残留的绿色分析方法。
本研究探讨了小型化QuEChERS方法在环境条件下粉碎三种果蔬中农药残留分析的可行性。菠菜、橙和红葡萄的实验室样品,含有12种喷洒农药,覆盖了广泛的物理化学性质和多达6种产生的农药,使用通用食品切割器/搅拌机(一步粉碎)或切割器/搅拌机的组合,然后使用UltraTurrax进一步均质(两步粉碎)进行粉碎。随后分别收集不同重量(15、2和0.5 g)的分析试验部分。称重后的测试部分用按比例缩小的乙腈和盐提取,用LC-MS/MS分析。结果显示,在分析结果中没有问题的可变性,即使在所有测试商品中喷洒和产生的农药的测试部分小至0.5 g。最佳粉碎方法取决于商品:虽然在大多数情况下一步粉碎就足够了,但某些商品需要两步粉碎才能为小型化提供足够的均匀性。QuEChERS方法可以缩小到0.5 g测试部分,无需进行低温研磨,使实验室能够进行更环保的农药残留分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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