Lead biosorption by Bacillus tequilensis FB-6: characterization of resistance mechanisms and its potential for remediation of contaminated soil.

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-06-02 DOI:10.1002/jsfa.14408

Xu Wang, Wei Xu, Yuling Lu, Lijuan Cao, Mengqing Tian, Yi Shang, Nanyu Han, Junmei Ding

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Abstract

Background: Lead (Pb) contamination in agricultural soils poses significant threats to food safety and environmental sustainability. Microbial bioremediation offers a sustainable solution, yet the mechanisms underlying microbial Pb tolerance and immobilization remain insufficiently explored. This study investigates the Pb resistance and adsorption characteristics of Bacillus tequilensis FB-6, a strain isolated from a Pb-contaminated site.

Results: B. tequilensis FB-6 displayed a high Pb²⁺ tolerance, with a minimum inhibitory concentration of 2200 mg L^-1, and exhibited cross-resistance to other heavy metals including Mn²⁺ and Cu²⁺. Microscopic and spectroscopic analyses indicated that Pb²⁺ binding was facilitated by extracellular polymeric substances (EPS), particularly polysaccharides, and that Pb²⁺ was converted into the insoluble mineral Pb₉(PO₄)₆. Enzyme activity assays revealed an increase in superoxide dismutase activity (15.5-47.5%) and a corresponding decrease in dehydrogenase activity (5.7-49.1%) under Pb²⁺ stress, suggesting oxidative stress response mechanisms. In plant co-cultivation trials, root inoculation with B. tequilensis FB-6 significantly reduced Pb uptake and enhanced growth performance, indicating its potential to mitigate Pb bioavailability in soil.

Conclusion: B. tequilensis FB-6 exhibits strong Pb tolerance and immobilization capacity, primarily through EPS-mediated biosorption and mineral precipitation. Its ability to reduce Pb bioavailability and support plant health underscores its promise as a biological tool for in situ remediation of Pb-contaminated agricultural soils. Further field-scale research is warranted to evaluate its practical applicability in diverse environmental contexts. © 2025 Society of Chemical Industry.

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龙舌兰芽孢杆菌FB-6对铅的生物吸附：抗性机制及其修复污染土壤的潜力

背景：农业土壤中的铅污染对食品安全和环境可持续性构成重大威胁。微生物生物修复提供了一种可持续的解决方案，但微生物耐铅和固定化的机制仍未得到充分探讨。研究了龙舌兰芽孢杆菌FB-6对铅的抗性和吸附特性。结果：B. tequilensis FB-6对Pb2+具有较高的耐受性，最低抑菌浓度为2200 mg L-1，对Mn2+、Cu2+等重金属具有交叉抗性。显微镜和光谱分析表明，细胞外聚合物（EPS），特别是多糖，促进了Pb2+的结合，并且Pb2+转化为不溶性矿物Pb9(PO4)6。酶活性测定显示，Pb2+胁迫下，超氧化物歧化酶活性升高（15.5-47.5%），脱氢酶活性降低（5.7-49.1%），提示氧化应激反应机制。在植物共栽培试验中，根系接种龙舌兰芽孢杆菌FB-6显著降低了铅的吸收，提高了生长性能，表明其可能降低土壤中铅的生物有效性。结论：B. tequilensis FB-6具有较强的铅耐受性和固定化能力，主要通过eps介导的生物吸附和矿物质沉淀。其降低铅生物利用度和支持植物健康的能力强调了其作为铅污染农业土壤原位修复的生物工具的前景。需要进一步的实地研究来评估其在不同环境背景下的实际适用性。©2025化学工业协会。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.