Shaoshan Wang , Yani Guo , Bingdi Wei , Rukun Lu , Zhixuan Tan , Chaojun Wei
{"title":"Dopaminergic neurons in the ventral periaqueductal gray projecting to the dorsal lateral septum regulate comorbid pain and anxiety","authors":"Shaoshan Wang , Yani Guo , Bingdi Wei , Rukun Lu , Zhixuan Tan , Chaojun Wei","doi":"10.1016/j.brainresbull.2025.111409","DOIUrl":null,"url":null,"abstract":"<div><div>The comorbidity of pain and anxiety is one of the most prevalent mental health disorders globally. However, its underlying etiological mechanisms remain incompletely understood. This study revealed that the dorsal lateral septum (LSD) and its associated neural circuits play key roles in pain and/or anxiety regulation. Using chemical genetic techniques, we found that the specific inhibition of LSD GABAergic neurons significantly alleviated pain responses and anxiety-like behaviors. Conversely, the specific activation of LSD GABAergic neurons induced hyperalgesia and anxiety-like behaviors in mice. Furthermore, our study showed that dopaminergic neurons in the ventral periaqueductal gray (vPAG) play a crucial role in regulating pain and anxiety comorbidity through their projections to the LSD. This regulatory mechanism depends on the release of dopamine and its binding to the D2 receptor of LSD. In summary, this study highlights the critical role of LSD GABAergic neurons and their associated neural circuits in the comorbidity of pain and anxiety, thereby providing a new theoretical foundation and research direction for developing potential therapeutic strategies.</div></div>","PeriodicalId":9302,"journal":{"name":"Brain Research Bulletin","volume":"228 ","pages":"Article 111409"},"PeriodicalIF":3.7000,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brain Research Bulletin","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0361923025002217","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
The comorbidity of pain and anxiety is one of the most prevalent mental health disorders globally. However, its underlying etiological mechanisms remain incompletely understood. This study revealed that the dorsal lateral septum (LSD) and its associated neural circuits play key roles in pain and/or anxiety regulation. Using chemical genetic techniques, we found that the specific inhibition of LSD GABAergic neurons significantly alleviated pain responses and anxiety-like behaviors. Conversely, the specific activation of LSD GABAergic neurons induced hyperalgesia and anxiety-like behaviors in mice. Furthermore, our study showed that dopaminergic neurons in the ventral periaqueductal gray (vPAG) play a crucial role in regulating pain and anxiety comorbidity through their projections to the LSD. This regulatory mechanism depends on the release of dopamine and its binding to the D2 receptor of LSD. In summary, this study highlights the critical role of LSD GABAergic neurons and their associated neural circuits in the comorbidity of pain and anxiety, thereby providing a new theoretical foundation and research direction for developing potential therapeutic strategies.
期刊介绍:
The Brain Research Bulletin (BRB) aims to publish novel work that advances our knowledge of molecular and cellular mechanisms that underlie neural network properties associated with behavior, cognition and other brain functions during neurodevelopment and in the adult. Although clinical research is out of the Journal''s scope, the BRB also aims to publish translation research that provides insight into biological mechanisms and processes associated with neurodegeneration mechanisms, neurological diseases and neuropsychiatric disorders. The Journal is especially interested in research using novel methodologies, such as optogenetics, multielectrode array recordings and life imaging in wild-type and genetically-modified animal models, with the goal to advance our understanding of how neurons, glia and networks function in vivo.