Enhanced Intracellular Delivery via Stainless Steel Filters and Viscoelastic Fluids: A High-Efficiency Alternative to Conventional Transfection

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2025-06-01 DOI:10.1021/acs.analchem.5c02251

Qiang Zhao, Partha Pratim Sarkar, Ye Ai

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Abstract

Advancements in intracellular delivery have garnered significant attention due to their potential applications in the biological and medical fields. Traditional methods, including virus infection, electroporation, and lipofection, have limitations such as toxicity, high costs, and low delivery efficiency. This study presents a novel intracellular delivery method utilizing a stainless-steel filter combined with poly(ethylene oxide) (PEO) solution to transport biomolecules into cells. Optimal parameters were identified through systematic adjustments using fluorescein isothiocyanate (FITC)-dextran. The performance was further evaluated by mRNA encoding enhanced green fluorescent protein (eGFP), demonstrating a remarkable delivery efficiency of 94.7%, a cell viability rate of 94.3%, and a throughput of 1 × 10⁷ cells/min. This new approach not only provides a cost-effective and user-friendly alternative but also enhances the potential for high-throughput applications in biological research and therapeutic interventions. The findings suggest that the integration of stainless-steel filters and viscoelastic fluids enhances intracellular delivery capabilities, positioning this method as a promising tool for advancing cellular engineering technologies.

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通过不锈钢过滤器和粘弹性流体增强细胞内传递：传统转染的高效替代方案

细胞内递送的进展由于其在生物和医学领域的潜在应用而引起了极大的关注。传统的方法，包括病毒感染、电穿孔和脂肪感染，存在毒性、成本高、输送效率低等局限性。本研究提出了一种新的细胞内递送方法，利用不锈钢过滤器结合聚环氧乙烷（PEO）溶液将生物分子运输到细胞内。采用异硫氰酸荧光素(FITC)-葡聚糖进行系统调整，确定最佳参数。通过mRNA编码增强绿色荧光蛋白（eGFP）进一步评估其性能，结果表明其递送效率为94.7%，细胞存活率为94.3%，吞吐量为1 × 107个细胞/min。这种新方法不仅提供了一种具有成本效益和用户友好的替代方法，而且还增强了生物研究和治疗干预中高通量应用的潜力。研究结果表明，不锈钢过滤器和粘弹性流体的集成增强了细胞内输送能力，使这种方法成为推进细胞工程技术的一种有前途的工具。

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.