Comparative evaluation of QuEChERS method with LC-MS/MS for three pesticides; novaluron, dimethomorph, and iprodione.

IF 2.2
Chun-Yeong Lee, MiSun Hong, Bo-Kyung Jung, Dong Kyu Kim, Yun-Jeong Yi, Mi-Sun Kim, HeeJin Choi, Min-Jeong Kim, Jin-Kyung Yu, Ha-Na Kwon, Su-Jin Jeon, Hyun Jeong Kim, Eun-Sun Yun, JuSung Park
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Abstract

This study aims to enhance the efficiency of field inspection centers, which require rapid testing and swift distribution control of non-compliant agricultural products. Currently, these centers in Korea use Multi-Residue Pesticide Test Method No. 2 to simultaneously analyse 510 pesticides. While 470 pesticides can be quantified using this method, others require additional quantification using individual test methods. Novaluron, dimethomorph, and iprodione, pesticides frequently exceed maximum residue limits, must be quantified using Individual Test Method No. 22 when detected by Method No. 2. This study validated and compared the effectiveness of these two methods for analysing the three pesticides in four agricultural matrices-lettuce, chili pepper, Welsh onion, and mandarin-by assessing specificity, linearity, detection and quantification limits, matrix effect, recovery rates, and repeatability. LC-MS/MS analysis showed both methods demonstrated high specificity and separation efficiency, with a coefficient of determination (R2 > 0.99). LOQ values for vegetables were below 0.01 mg/kg, meeting regulatory standards; however, for mandarin, Method No. 2 exceeded the limit (0.02 mg/kg), while Method No. 22 remained within range. For vegetables recovery rates were slightly better with Method No. 22, but met validation criteria for both methods. In mandarin, recovery rates did not meet the validation criteria with Method No. 2. Novaluron at 0.02 mg/kg exceeded the acceptable RSD limit (23% vs. 20%), while iprodione showed slightly elevated RSD but remained acceptable. All other results met validation criteria. In conclusion, Method No. 2 was validated for quantifying the three pesticides in vegetables, demonstrating its potential application in field inspections. Monitoring of 1,744 agricultural samples revealed seven cases (0.4%) exceeding residue limits, including dimethomorph in basil (3 cases) and coriander (1 case) and iprodione in carrot (2 cases) and chard (1 case). Additionally, 46 cases (2.6%) were detected within acceptable limits, including novaluron (2 cases), dimethomorph (42 cases), and iprodione (2 cases).for mandarin samples did not meet the criteria in Method No. 2.

QuEChERS法与LC-MS/MS法测定3种农药的比较评价诺伐龙,吡虫啉和异丙酮。
本研究旨在提高现场检验中心的效率,因为现场检验中心需要对不合格农产品进行快速检测和快速配送控制。目前,国内的这些中心正在对510种农药进行同时分析,使用的是多残留农药检验方法2号。虽然使用这种方法可以对470种农药进行量化,但其他农药需要使用个别测试方法进行额外的量化。诺伐威龙、啶虫啉和异丙醇等农药经常超过最大残留限量,在用方法2检测时,必须用单独试验方法22进行定量。本研究通过评估特异性、线性度、检测和定量限、基质效应、回收率和重复性,验证并比较了这两种方法在莴苣、辣椒、威尔士洋葱和橘子四种农业基质中三种农药分析的有效性。LC-MS/MS分析表明,两种方法均具有较高的特异性和分离效率,测定系数(R2 > 0.99)。蔬菜的LOQ值低于0.01 mg/kg,符合规定标准;方法2超标(0.02 mg/kg),而方法22在范围内。方法22对蔬菜的回收率稍好,但满足两种方法的验证标准。在普通话中,回收率不符合方法2的验证标准。诺伐龙0.02 mg/kg超过可接受的RSD限值(23%对20%),而异丙地酮的RSD略有升高,但仍可接受。所有其他结果均符合验证标准。综上所述,方法2可用于蔬菜中三种农药的定量分析,具有一定的应用前景。1744份农用样品中残留超标7份(0.4%),其中罗勒(3份)、香菜(1份)中残留啶虫啉超标,胡萝卜(2份)和甜菜(1份)中残留异丙二酮超标。在可接受范围内检出46例(2.6%),包括新伐脲(2例)、啶虫啉(42例)和异丙砜(2例)。的样品不符合方法2的标准。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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