Antimicrobial Susceptibility Profiles of Staphylococcus aureus Isolates from Domestic Pigeons in Hungary in 2022.

IF 4.3 2区 医学 Q1 INFECTIOUS DISEASES
Ádám Kerek, Ábel Szabó, Ákos Jerzsele
{"title":"Antimicrobial Susceptibility Profiles of <i>Staphylococcus aureus</i> Isolates from Domestic Pigeons in Hungary in 2022.","authors":"Ádám Kerek, Ábel Szabó, Ákos Jerzsele","doi":"10.3390/antibiotics14050525","DOIUrl":null,"url":null,"abstract":"<p><p><b>Background</b>: Antimicrobial resistance (AMR) is a critical global health threat, affecting both human and veterinary medicine. Pigeons are increasingly recognized as potential reservoirs of antibiotic-resistant bacteria due to their widespread presence in urban and rural environments. The aim of this study was to determine the antimicrobial susceptibility profiles of <i>Staphylococcus aureus</i> isolates from pigeons in Hungary. <b>Methods</b>: A total of 73 <i>S. aureus</i> isolates were collected from pigeons across seven regions of Hungary in 2022. Minimum inhibitory concentrations (MICs) were determined using the broth microdilution method according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Statistical analysis included correlation heatmaps, hierarchical clustering, network analysis, decision tree modeling, and Monte Carlo simulations. <b>Results</b>: The multidrug-resistant (MDR) prevalence rate was alarmingly high at 80.8%. Very high resistance rates were observed for doxycycline (97.3%), enrofloxacin (87.7%), and amoxicillin (84.9%). By contrast, low resistance rates were detected for vancomycin (5.5%) and imipenem (8.2%). Decision tree modeling identified tiamulin, enrofloxacin, and amoxicillin-clavulanate resistance as the most significant predictors of MDR status. Monte Carlo simulations predicted a mean MDR prevalence of 78.5%, indicating that the dominance of MDR strains is not merely a random phenomenon but part of a broader epidemiological pattern. <b>Conclusions</b>: These findings confirm that pigeons may serve as critical reservoirs of MDR <i>S. aureus</i> strains, posing a potential risk to public and animal health. Continued monitoring, the genetic characterization of resistant strains, and the development of effective control strategies are urgently needed. This study provides a foundation for future research aimed at understanding the biological, ecological, and epidemiological roles of pigeon-associated MDR strains.</p>","PeriodicalId":54246,"journal":{"name":"Antibiotics-Basel","volume":"14 5","pages":""},"PeriodicalIF":4.3000,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12108451/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antibiotics-Basel","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3390/antibiotics14050525","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Antimicrobial resistance (AMR) is a critical global health threat, affecting both human and veterinary medicine. Pigeons are increasingly recognized as potential reservoirs of antibiotic-resistant bacteria due to their widespread presence in urban and rural environments. The aim of this study was to determine the antimicrobial susceptibility profiles of Staphylococcus aureus isolates from pigeons in Hungary. Methods: A total of 73 S. aureus isolates were collected from pigeons across seven regions of Hungary in 2022. Minimum inhibitory concentrations (MICs) were determined using the broth microdilution method according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Statistical analysis included correlation heatmaps, hierarchical clustering, network analysis, decision tree modeling, and Monte Carlo simulations. Results: The multidrug-resistant (MDR) prevalence rate was alarmingly high at 80.8%. Very high resistance rates were observed for doxycycline (97.3%), enrofloxacin (87.7%), and amoxicillin (84.9%). By contrast, low resistance rates were detected for vancomycin (5.5%) and imipenem (8.2%). Decision tree modeling identified tiamulin, enrofloxacin, and amoxicillin-clavulanate resistance as the most significant predictors of MDR status. Monte Carlo simulations predicted a mean MDR prevalence of 78.5%, indicating that the dominance of MDR strains is not merely a random phenomenon but part of a broader epidemiological pattern. Conclusions: These findings confirm that pigeons may serve as critical reservoirs of MDR S. aureus strains, posing a potential risk to public and animal health. Continued monitoring, the genetic characterization of resistant strains, and the development of effective control strategies are urgently needed. This study provides a foundation for future research aimed at understanding the biological, ecological, and epidemiological roles of pigeon-associated MDR strains.

2022年匈牙利家鸽金黄色葡萄球菌的药敏分析
背景:抗微生物药物耐药性(AMR)是一个严重的全球健康威胁,影响着人类和兽药。由于鸽子广泛存在于城市和农村环境中,人们越来越认识到鸽子是抗生素耐药细菌的潜在宿主。本研究的目的是确定从匈牙利鸽子分离的金黄色葡萄球菌的抗菌敏感性。方法:在2022年从匈牙利7个地区的鸽子中采集了73株金黄色葡萄球菌分离株。根据临床和实验室标准协会(CLSI)的指南,采用肉汤微量稀释法测定最低抑菌浓度(mic)。统计分析包括相关热图、分层聚类、网络分析、决策树建模和蒙特卡罗模拟。结果:多药耐药(MDR)患病率高达80.8%。多西环素(97.3%)、恩诺沙星(87.7%)和阿莫西林(84.9%)的耐药率很高。万古霉素(5.5%)和亚胺培南(8.2%)的耐药率较低。决策树模型确定了替穆林、恩诺沙星和阿莫西林-克拉维酸耐药是耐多药状态最重要的预测因子。蒙特卡罗模拟预测MDR的平均流行率为78.5%,表明MDR菌株的优势不仅仅是一种随机现象,而是更广泛的流行病学模式的一部分。结论:这些发现证实,鸽子可能是耐多药金黄色葡萄球菌菌株的关键宿主,对公众和动物健康构成潜在风险。迫切需要继续进行监测,鉴定耐药菌株的遗传特征,并制定有效的控制策略。本研究为进一步了解鸽相关耐多药菌株的生物学、生态学和流行病学作用奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Antibiotics-Basel
Antibiotics-Basel Pharmacology, Toxicology and Pharmaceutics-General Pharmacology, Toxicology and Pharmaceutics
CiteScore
7.30
自引率
14.60%
发文量
1547
审稿时长
11 weeks
期刊介绍: Antibiotics (ISSN 2079-6382) is an open access, peer reviewed journal on all aspects of antibiotics. Antibiotics is a multi-disciplinary journal encompassing the general fields of biochemistry, chemistry, genetics, microbiology and pharmacology. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of papers.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信