Combined Herbal Eye Drops Exhibit Neuroprotective and Intraocular Pressure-Reducing Effects in a Glaucoma Rat Model.

IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Tibor Rak, Evelin Patko, Edina Szabo, Alexandra Vaczy, Dorottya Molitor, Dora Reglodi, Adrienne Csutak, Tamas Atlasz
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引用次数: 0

Abstract

(1) Background: Glaucoma is a multifactorial group of diseases characterized by progressive optic neuropathy. Intraocular pressure (IOP) is the only successfully modifiable risk factor for all forms of glaucoma. However, recent research has highlighted the reduction of oxidative stress and neuroinflammation as promising therapeutic targets. In this study, we evaluated the antiglaucomatous effects of a combined herbal extract applied as eye drops in a rat model of glaucoma. (2) Methods: Sprague Dawley rats were divided into four groups: healthy controls, glaucomatous animals treated with preservative-free artificial tears, and healthy and glaucomatous groups receiving combined herbal-based eye drops for 8 weeks. Glaucoma was induced through injection of microbeads into the anterior chamber at week 1 and week 3. Before the first injection and at weeks 4 and 8, rats underwent optical coherence tomography (OCT) and electroretinogram (ERG) recordings. Retinal analyses were conducted to assess retinal ganglion cell (RGC) count, vessel density, and markers of neural pathways, oxidative stress, and inflammation. (3) Results: The combination of herbal extracts showed beneficial effects on IOP elevation, and significantly improved ERG responses. Neuroprotective effects were assessed using OCT, immunohistochemistry, and proteomics. Most parameters in herbal eye drop-treated rats were not statistically different from those in healthy controls. (4) Conclusions: Topical administration of plant-based compounds may serve as an effective supportive therapy for ocular hypertension and retinal neuroprotection.

复方草药滴眼液在青光眼大鼠模型中具有神经保护和眼压降低作用。
(1)背景:青光眼是一组以进行性视神经病变为特征的多因素疾病。眼内压(IOP)是所有形式青光眼唯一可成功改变的危险因素。然而,最近的研究强调,减少氧化应激和神经炎症是有希望的治疗靶点。在这项研究中,我们评估了联合草药提取物作为眼药水应用于青光眼大鼠模型的抗青光眼效果。(2)方法:将Sprague Dawley大鼠分为4组:健康对照组,青光眼动物给予不含防腐剂的人工泪液治疗,健康组和青光眼动物给予复方滴眼液治疗,疗程8周。分别于第1周和第3周采用前房注射微珠诱导青光眼。在第一次注射前和第4周和第8周,对大鼠进行光学相干断层扫描(OCT)和视网膜电图(ERG)记录。进行视网膜分析以评估视网膜神经节细胞(RGC)计数、血管密度、神经通路、氧化应激和炎症标志物。(3)结果:中药提取物联合用药对IOP升高有一定的促进作用,并显著改善ERG反应。使用OCT、免疫组织化学和蛋白质组学评估神经保护作用。中药滴眼液治疗大鼠的大部分参数与健康对照无统计学差异。(4)结论:局部给药植物性化合物可能是高眼压和视网膜神经保护的有效支持疗法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Antioxidants
Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍: Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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