Obtainment of Flavonoid-Enriched Fractions from Maqui (Aristotelia chilensis) and Murta (Ugni molinae) Extracts via Preparative HPLC and Evaluation of Their Anti-Inflammatory Effects in Cell-Based Assays.

IF 6 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Antioxidants Pub Date : 2025-05-16 DOI:10.3390/antiox14050600

Amador Alburquenque, Carolina Busch, Gabriela Gómez-Lillo, Alexander Gamboa, Camilo Perez, Nelson Caro Fuentes, Martin Gotteland, Lilian Abugoch, Cristian Tapia

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引用次数: 0

Abstract

Polyphenols exert anti-inflammatory and antioxidant effects by modulating cell signalling pathways and transcription factors involved in inflammatory bowel disease (IBD). However, their stability during digestion can be compromised. Polymer coatings like chitosan (-C) help preserve their stability. Maqui (Aristotelia chilensis) and murta (Ugni molinae) are rich in antioxidant and anti-inflammatory compounds. This work aims to obtain extracts (E) and blends (B) enriched in delphinidin and quercetin glucosides from maqui (Ma) and murta (Mu) crude extracts using preparative chromatography methodology (Prep-HPLC) and to evaluate their effectiveness through in vitro and cellular assays. HPLC-DAD analysis revealed a marked increase in phenolic compound concentration in the BEMaMu and BCMaMu extract blends. Total quercetin glycosides (TQG) increased by 11-fold, and total anthocyanins increased by approximately 8-fold compared to the fruit blend (BMaMu). BCMaMu exhibited a significantly higher ORAC value compared to the estimated additive mixture, suggesting a synergistic effect. No cytotoxicity was observed for BEMaMu, BCMaMu, and their chitosan-coated versions (BEMaMu-C and BCMaMu-C) in Caco-2 and HT29-MTX-E12 cells at concentrations of 0.1-50 mg/mL. Notably, only chitosan-coated BCMaMu inhibited NF-κB expression and activated Nrf2 in TNF-α-challenged Caco-2 cells at 0.1 and 0.5 mg/mL.

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利用制备高效液相色谱法从黄芪（亚里斯多德）和木犀草（亚里斯多德）提取物中提取富含黄酮类化合物，并在细胞基础上评价其抗炎作用。

多酚通过调节参与炎症性肠病（IBD）的细胞信号通路和转录因子发挥抗炎和抗氧化作用。然而，它们在消化过程中的稳定性可能会受到损害。壳聚糖(c)等聚合物涂层有助于保持它们的稳定性。马基（亚里斯多德）和穆塔（Ugni molinae）富含抗氧化和抗炎化合物。利用制备色谱法（prepare - hplc）从马基（Ma）和木犀草（Mu）粗提物中获得富含飞鸽苷和槲皮素糖苷的提取物(E)和共混物(B)，并通过体外和细胞实验评价其有效性。HPLC-DAD分析显示，BEMaMu和BCMaMu提取物混合物中酚类化合物浓度显著增加。总槲皮素苷（TQG）增加了11倍，总花青素增加了约8倍的水果混合物（BMaMu）。BCMaMu的ORAC值明显高于预估的添加剂混合物，表明其具有协同效应。在0.1 ~ 50mg /mL浓度下，对Caco-2和HT29-MTX-E12细胞，BEMaMu、BCMaMu及其壳聚糖包被版本（BEMaMu- c和BCMaMu- c）均无细胞毒性。值得注意的是，只有壳聚糖包被的BCMaMu在0.1和0.5 mg/mL时抑制TNF-α-挑战的Caco-2细胞中NF-κB的表达并激活Nrf2。

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来源期刊

Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology

CiteScore

10.60

自引率

11.40%

发文量

2123

审稿时长

16.3 days

期刊介绍： Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.