Green Approach for Rosa damascena Mill. Petal Extract: Insights into Phytochemical Composition, Anti-Aging Potential, and Stability.

IF 6 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Antioxidants Pub Date : 2025-04-30 DOI:10.3390/antiox14050541

Sawat Sopharadee, Jutinat Kittipitchakul, Nutnaree Srisawas, Waranya Neimkhum, Artit Yawootti, Thomas Rades, Wantida Chaiyana

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引用次数: 0

Abstract

Rosa damascena Mill., widely recognized for its remarkable skincare benefits, is extensively used in the cosmeceutical industry. This study introduces a novel green approach to extract bioactive compounds from R. damascena for cosmeceutical applications while also evaluating its stability in terms of physical, chemical, and biological properties. R. damascena petals were extracted using deionized water instead of organic solvents, using various green extraction methods, including infusion, microwave, ultrasound, pulsed electric field, and micellar extraction. Their chemical composition was analyzed using high-performance liquid chromatography. The extract with the highest concentration of bioactive compounds was further evaluated for its cosmeceutical properties and stability and compared with its individual chemical components. Various factors influencing stability were evaluated, including pH level (5, 7, and 9), temperature (4 °C, 30 °C, and 45 °C), and light exposure. The findings indicate that the extract obtained through microwave-assisted extraction (MAE) contained the highest concentration of bioactive constituents, with corilagin being the most abundant, followed by cyanidin-3,5-O-diglucoside, gallic acid, ellagic acid, L-ascorbic acid, and rutin, respectively. Additionally, MAE exhibited excellent antioxidant, whitening, and anti-skin-aging effects, demonstrating significantly higher activities than both the positive control (L-ascorbic acid for antioxidant effects, kojic acid for anti-tyrosinase effects, and epigallocatechin gallate and oleanolic acid for anti-skin-aging effects) and the individual chemical constituents. However, the physico-chemical and biological stability of MAE was influenced by pH, temperature, and light exposure, and as such, light-protected and controlled temperature (not exceeding 30 °C) is essential to maintain the extract's efficacy in skincare products, and optimal formulation strategies are strongly recommended to ensure long-term stability.

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Rosa damascena Mill的绿色方法。花瓣提取物：植物化学成分，抗衰老潜力和稳定性的见解。

玫瑰大马士革磨坊。，因其显著的护肤功效而被广泛认可，被广泛用于药妆工业。本研究介绍了一种新的绿色方法从大马花中提取药妆用的生物活性化合物，并从物理、化学和生物特性方面评价了大马花的稳定性。采用去离子水代替有机溶剂提取大马花花瓣，采用灌注、微波、超声、脉冲电场、胶束提取等多种绿色提取方法。采用高效液相色谱法对其化学成分进行了分析。进一步评价了生物活性化合物浓度最高的提取物的药妆性能和稳定性，并与其单个化学成分进行了比较。评估了影响稳定性的各种因素，包括pH值（5、7和9）、温度（4°C、30°C和45°C）和光照。结果表明，微波辅助提取（MAE）的提取液中活性成分含量最高，其中花青素含量最高，花青素-3,5- o -二糖苷含量次之，没食子酸含量次之，鞣花酸含量次之，l -抗坏血酸含量次之，芦丁含量次之。此外，MAE具有优异的抗氧化、美白和抗皮肤衰老作用，其活性显著高于阳性对照（l -抗坏血酸具有抗氧化作用，曲酸具有抗酪氨酸酶作用，表没食子儿茶素没食子酸酯和齐ole果酸具有抗皮肤衰老作用）和单个化学成分。然而，MAE的物理化学和生物稳定性受到pH、温度和光照的影响，因此，遮光和控制温度（不超过30°C）对于保持提取物在护肤品中的功效至关重要，强烈建议优化配方策略以确保长期稳定性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology

CiteScore

10.60

自引率

11.40%

发文量

2123

审稿时长

16.3 days

期刊介绍： Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.