Rapid and sensitive in-situ detection of malachite green and crystal violet in aquaculture using flexible SERS substrates via instant self-assembly

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-05-27 DOI:10.1016/j.foodchem.2025.144920

Yupeng Sun , Kaihui Zhou , Yuxin Wang , Ye Lin , Weiran Wang , Xue Xiong , Xiangru Zhao , Huinan Cui , Min Liang , Qiao Wang , Shengnan Yang , Li Li

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Abstract

Rapid, low-cost, and in-situ detection of illegal residues on aquatic product surfaces remains a significant challenge. Herein, we developed a versatile surface-enhanced Raman scattering (SERS) sensor by integrating instant interfacial self-assembled silver nanostar film onto flexible polytetrafluoroethylene filter membrane (AgNS/PTFE) for sensitive detection of malachite green (MG) and crystal violet (CV) in crayfish. The entire process, from room-temperature substrate preparation to detection completion, takes less than 20 min. The AgNS/PTFE flexible substrate not only provides excellent hydrophobicity for signal enhancement with minimal sample volumes (2 μL) but also ensures good uniformity, resulting in a homogeneous and dense distribution of SERS-active hot spots with superior anti-interference properties and mechanical durability. By simple “drop/wipe” operations and data visualization treatment, this method achieved detection limits as low as 10 pg/mL for MG and 7 pg/mL for CV, with in-situ analysis in spiked and actual crayfish samples, demonstrating significant potential for food safety applications.

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基于即时自组装的柔性SERS基底快速灵敏地原位检测水产养殖中的孔雀石绿和结晶紫

快速、低成本和原位检测水产品表面的非法残留仍然是一个重大挑战。在此，我们将即时界面自组装银纳米星膜集成到柔性聚四氟乙烯过滤膜（AgNS/PTFE）上，开发了一种多功能表面增强拉曼散射（SERS）传感器，用于灵敏检测小龙虾中的孔雀石绿（MG）和结晶紫（CV）。从室温基板制备到检测完成，整个过程耗时不到20 min。AgNS/PTFE柔性衬底不仅具有优异的疏水性，以最小的样品体积（2 μL）增强信号，而且保证了良好的均匀性，导致sers活性热点均匀密集分布，具有优异的抗干扰性能和机械耐久性。通过简单的“滴/擦”操作和数据可视化处理，该方法对MG的检出限低至10 pg/mL，对CV的检出限低至7 pg/mL，可在加标和实际小龙虾样品中进行原位分析，显示出在食品安全方面的巨大应用潜力。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.