How mechanical pre-treatment affects the extraction yield, nutritional, structural, and functional properties of leaf proteins

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-05-27 DOI:10.1016/j.foodchem.2025.144934

Ram Prasad Bebartta, Rachna Sehrawat, Khalid Gul, Ankan Kheto

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Abstract

Moringa leaves, rich in functional proteins, may benefit from ball milling (BM) to enhance protein extraction and functionality through structural modification. BM for 3 h resulted in highest protein yield (180 %) and improved digestibility. Though 5 h treatment increased the extraction yield, it reduced protein purity. The BM significantly reduced β-sheet (51.5 to 42.1 %) and increased random coil structures (23.3 to 34.4 %), suggesting protein unfolding. Except at 5 h, increasing BM duration reduced the disulfide bonds, denaturation temperature, enthalpy, surface hydrophobicity, and particle sizes of moringa leaf protein concentrate (MLPC). Similarly, solubility, water holding capacity, and foaming capacity were also significantly improved in MLPC up to 3 h of BM. Correlation analysis indicated that BM duration significantly affected the essential amino acids, carbonyl content, sulfhydryl groups, and functional properties of MLPC. Conclusively, moderate BM duration (3 h) underscores its potential as a scalable pre-treatment for producing high-quality plant protein ingredients.

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机械预处理如何影响叶蛋白的提取率、营养、结构和功能特性

辣木叶富含功能蛋白，球磨可以通过结构修饰来提高蛋白质的提取和功能。发酵3 h蛋白质产量最高（180 %），消化率提高。5 h处理虽然提高了提取率，但降低了蛋白纯度。BM显著减少了β-sheet(51.5 - 42.1 %)，增加了随机线圈结构（23.3 - 34.4 %），提示蛋白质展开。除5 h外，保温时间的延长降低了辣木叶蛋白浓缩物的二硫键、变性温度、变性焓、表面疏水性和粒径。同样，当BM浓度达到3 h时，MLPC的溶解度、保水能力和发泡能力也显著提高。相关分析表明，保温时间显著影响了MLPC的必需氨基酸、羰基含量、巯基和功能特性。最后，适度的BM持续时间（3 h）强调了其作为生产高质量植物蛋白成分的可扩展预处理的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.