High-sensitivity Ara h1 detection with a praseodymium niobate-carbon nanofiber immunosensor

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-05-27 DOI:10.1016/j.foodchem.2025.144922

Evangeline Jafneel Nixon , Rajalakshmi Sakthivel , Yu-Chien Lin , Hsiao-Wei Wen , Ching-Wei Tung , Ren-Jei Chung

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引用次数: 0

Abstract

Peanut allergy is a common and potentially life-threatening condition. To address this, an electrochemical immunosensor was developed for detecting the peanut allergen Ara h 1. The sensor uses gold electrodes modified with PrNbO₄/f-CNF in a label-free immunoassay. Monoclonal capture and Anti-Ara h1 antibodies ensure high specificity. A pre-incubation step accelerates detection, making it suitable for real-time monitoring. Differential pulse voltammetry (DPV) provides precise measurements. The sensor has a linear detection range of 0.0062 to 620 fg·mL⁻¹ and a detection limit of 0.0029 fg·mL⁻¹. It demonstrates high sensitivity (5.97 μA·mL⁻¹·fg·cm⁻²) and precision (Vx0 ∼ 6 %). Tests with commercial products confirmed detection at 0.05 % (m/m) peanut. These results highlight the sensor's reliability and potential application in food safety, ensuring even trace amounts of allergens are identified.

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利用铌镨-碳纳米纤维免疫传感器进行高灵敏度Ara h1检测

花生过敏是一种常见且可能危及生命的疾病。为了解决这一问题，研制了一种用于检测花生过敏原Ara h1的电化学免疫传感器。该传感器在无标记免疫分析中使用PrNbO4/f-CNF修饰的金电极。单克隆捕获和抗ara h1抗体确保高特异性。预孵育步骤加速检测，使其适合实时监测。差分脉冲伏安法（DPV）提供精确的测量。传感器线性检测范围为0.0062 ~ 620 fg·mL−1，检出限为0.0029 fg·mL−1。灵敏度（5.97 μA·mL−1·fg·cm−2）、精密度（Vx0 ~ 6 %）高。对商业产品的检测证实为0.05 % （m/m）花生。这些结果突出了传感器的可靠性和在食品安全方面的潜在应用，确保即使是微量的过敏原也能被识别出来。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.