Novel receptor-binding protein from phage PJNS004 for sensitive detection of Salmonella

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-05-27 DOI:10.1016/j.foodchem.2025.144921

Yibao Chen , Simin Yang , Qing Zhang , Ming Hu , Qianghua Lv , Xiaonan Zhao , Lulu Li , Yanbo Luo , Xiaohui Xu , Yujun Zhao , Yuqing Liu

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Abstract

The detection of Salmonella, a key foodborne pathogen, is essential for maintaining food safety and safeguarding public health. Here, we have identified a novel receptor-binding protein (RBP), encoded by ORF33 of phage PJNS004, that specifically targets lipopolysaccharide on the host strain surface. To utilize this RBP for Salmonella detection, we expressed and purified the recombinant protein, designated as proSM and proM. Based on the principle of ELISA double antibody sandwich method, our developed two-step method boasts a detection limit of 10 CFU/mL and a rapid binding time of 30 min. The reliability and sensitivity of our method were further confirmed in food (lettuce, chicken breast, and milk) and clinical samples, with recovery rates ranging from 98 to 105 % and 85 to 115 %, respectively. In conclusion, our study presents a novel RBP from PJNS004 and a sensitive detection method for Salmonella, offering a valuable tool for food safety and clinical diagnostics.

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新型沙门氏菌噬菌体PJNS004受体结合蛋白的灵敏检测

沙门氏菌是一种重要的食源性病原体，检测沙门氏菌对维护食品安全和保障公众健康至关重要。在这里，我们鉴定了一种新的受体结合蛋白（RBP），由噬菌体PJNS004的ORF33编码，专门针对宿主菌株表面的脂多糖。为了利用该RBP检测沙门氏菌，我们表达并纯化了重组蛋白，分别命名为proSM和proM。基于ELISA双抗体夹心法原理，两步法检出限为10 CFU/mL，快速结合时间为30 min。在食品（生菜、鸡胸肉和牛奶）和临床样品中进一步验证了该方法的可靠性和灵敏度，回收率分别为98 ~ 105 %和85 ~ 115 %。本研究为PJNS004提供了一种新的RBP和一种灵敏的沙门氏菌检测方法，为食品安全和临床诊断提供了有价值的工具。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.