Anti-osteoporotic effects of peptide PASTGAAK derived from black-boned silky fowl on ovariectomized mice

IF 4.8 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience Pub Date : 2025-05-21 DOI:10.1016/j.fbio.2025.106908

Yang Qiu , Jiaxin Guo , Jie Zhang , Chenxu Xue , Wanjun He , Jiajin Zhu

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Abstract

This study investigated the anti-osteoporotic effects of the peptide PASTGAAK (P11) on ovariectomized (OVX) mice. In vitro gastrointestinal stability assays demonstrated that P11 resisted enzymatic degradation by trypsin and pepsin, retaining its osteogenic activity post-digestion. In vivo, P11 administration (8 weeks) significantly reversed OVX-induced bone loss, as evidenced by improved trabecular bone microstructure, as well as the enhanced biomechanical properties. Serum analysis revealed P11 was beneficial for upregulating bone formation markers. Gene expression profiling indicated P11 stimulated osteogenic differentiation via upregulation of Runx2, Osterix, ALP and Col1a1. Gut microbiota analysis showed P11 restored OVX-induced reductions in microbial richness, modulated the Firmicutes/Bacteroidota ratio and increased key genera (Akkermansia). Serum metabolomics identified P11-mediated restoration of lipid metabolism (α-linolenic acid pathway) and inhibition of serotonin synthesis. Correlation analysis revealed that the aforementioned metabolic changes induced by P11 were closely associated with the enrichment of Akkermansia in the gut. These findings highlighted P11 as a safe natural agent for osteoporosis treatment, acting via osteogenic promotion, gut microbiota modulation and metabolic pathway regulation.

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黑骨丝鸡肽PASTGAAK对去卵巢小鼠的抗骨质疏松作用

本研究探讨肽PASTGAAK （P11）对去卵巢小鼠的抗骨质疏松作用。体外胃肠稳定性实验表明，P11可以抵抗胰蛋白酶和胃蛋白酶的酶降解，在消化后保持其成骨活性。在体内，P11给药（8周）可显著逆转ovx诱导的骨质流失，这可以通过改善骨小梁微观结构和增强生物力学性能来证明。血清分析显示P11有利于上调骨形成标志物。基因表达谱显示P11通过上调Runx2、Osterix、ALP和Col1a1来促进成骨分化。肠道菌群分析显示，P11恢复了ovx诱导的微生物丰富度下降，调节了厚壁菌门/拟杆菌门比例，增加了关键属（Akkermansia）。血清代谢组学鉴定p11介导的脂质代谢（α-亚麻酸途径）恢复和血清素合成抑制。相关分析显示，P11诱导的上述代谢变化与肠道中Akkermansia的富集密切相关。这些发现强调P11是一种安全的天然骨质疏松治疗药物，通过促进成骨、调节肠道微生物群和调节代谢途径发挥作用。

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来源期刊

Food Bioscience Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

6.40

自引率

5.80%

发文量

671

审稿时长

27 days

期刊介绍： Food Bioscience is a peer-reviewed journal that aims to provide a forum for recent developments in the field of bio-related food research. The journal focuses on both fundamental and applied research worldwide, with special attention to ethnic and cultural aspects of food bioresearch.