Ginseng oligopeptide alleviates glycolipid metabolism disorders in db/db mice by regulating oxidative stress, inflammatory response and gut microbiota disorder

IF 4.8 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience Pub Date : 2025-05-22 DOI:10.1016/j.fbio.2025.106905

Tianzhu Liu , Chenhui Zhu , Pei Ma , Xiaoxuan Ma , Daidi Fan

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引用次数: 0

Abstract

Ginseng oligopeptide (GOP) have been shown to have hypoglycemic and hypolipidemic effects, although their mechanisms of action are not clear. In this study, we investigated the effects of different concentrations of GOP (30, 60 mg/kg) on glucose and lipid metabolism in male db/db mice (administered by tube-feeding method for 6 weeks). The results showed that GOP supplementation reduced fasting blood glucose levels (24.38–12.37 mM, P<0.01), alleviated insulin resistance, lowered triglycerides (2.85–1.66 mM, P<0.01), total cholesterol (5.75–3.21 mM, P<0.01), and low-density lipoprotein (0.53–0.26 mM, P<0.01), and increased high-density lipoprotein level (1.30–1.74 mM, P<0.05). Meanwhile, GOP could improve the intestinal structure, repair the damaged barrier injury, attenuate the histopathological changes in the liver, reduce the oxidative stress and pro-inflammatory cytokine expression in the liver, and repair the damaged intestinal microbiota, especially the abundance of Bacteroidetes and Bacillota in db/db mice (P<0.01). Further studies revealed that the regulatory effects of GOP on glucose and lipid metabolism can be achieved by acting on the intestinal microbiota and the AMPK signaling pathway. This study is the first to link GOP with AMPK signaling and changes in the gut microbiota, providing a theoretical basis for GOP as a functional food for the prevention and management of metabolic disorders.

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人参寡肽通过调节氧化应激、炎症反应和肠道菌群紊乱来缓解db/db小鼠糖脂代谢紊乱

人参寡肽（GOP）已被证明具有降血糖和降血脂的作用，但其作用机制尚不清楚。在本研究中，我们研究了不同浓度的GOP（30、60 mg/kg）对雄性db/db小鼠糖脂代谢的影响（管饲6周）。结果表明：添加GOP可降低空腹血糖水平（24.38 ~ 12.37 mM, P<0.01），缓解胰岛素抵抗，降低甘油三酯水平（2.85 ~ 1.66 mM, P<0.01）、总胆固醇水平（5.75 ~ 3.21 mM, P<0.01）、低密度脂蛋白水平（0.53 ~ 0.26 mM, P<0.01），升高高密度脂蛋白水平（1.30 ~ 1.74 mM, P<0.05）。同时，GOP可以改善肠道结构，修复受损屏障损伤，减轻肝脏组织病理改变，降低肝脏氧化应激和促炎细胞因子表达，修复受损肠道微生物群，特别是db/db小鼠的Bacteroidetes和Bacillota丰度（P<0.01）。进一步研究发现，GOP对糖脂代谢的调节作用可通过作用于肠道菌群和AMPK信号通路来实现。本研究首次将GOP与AMPK信号和肠道菌群变化联系起来，为GOP作为功能食品预防和管理代谢紊乱提供了理论依据。

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来源期刊

Food Bioscience Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

6.40

自引率

5.80%

发文量

671

审稿时长

27 days

期刊介绍： Food Bioscience is a peer-reviewed journal that aims to provide a forum for recent developments in the field of bio-related food research. The journal focuses on both fundamental and applied research worldwide, with special attention to ethnic and cultural aspects of food bioresearch.