Simultaneous detection of two pesticides by colorimetric and fluorescence dual-response sensor based on aptamer and gold nanoparticles

IF 4.6 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis Pub Date : 2025-05-12 DOI:10.1016/j.jfca.2025.107762

Yao He , Chunlei Lv , Fulin Li , Yichen Li , Xiangyue Tian , Xi Chen , Qiyu Yang , Juan Su , Daichen Mu

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Abstract

We have designed a sensor based on gold nanoparticles (AuNPs) and aptamers for two pesticides detection. Two complementary paired aptamers have been carefully designed to adsorb onto the surface of AuNPs, serving as binding elements and molecular switches for pesticides. Aptamers not only give AuNPs the ability of colorimetric detection, but also has the ability of fluorescence detection, and AuNPs can improve the stability of aptamers. Thiamethoxam (TMX) and Acetamiprid (ACE) and their corresponding aptamers (Tapt and Aapt) were selected as models. When TMX is added, Tapt can bind to it and trigger the aggregation of AuNPs which can be used as the detection signal of colorimetric detection. When ACE is added, Aapt can bind to it and produce fluorescence. ACE does not cause colorimetric reaction, and TMX does not cause fluorescence. By using both colorimetric and fluorescent detection methods, interference free detection of two targets can be achieved.

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基于适配体和金纳米粒子的比色和荧光双响应传感器同时检测两种农药

我们设计了一种基于金纳米粒子（AuNPs）和适配体的传感器，用于两种农药的检测。两个互补的配对适配体被精心设计以吸附在AuNPs表面，作为农药的结合元件和分子开关。适配体不仅使AuNPs具有比色检测能力，还具有荧光检测能力，并且AuNPs可以提高适配体的稳定性。选择噻虫嗪（TMX）和啶虫脒（ACE）及其对应的适体（Tapt和Aapt）作为模型。加入TMX后，Tapt与TMX结合，触发aunp聚集，作为比色检测的检测信号。当ACE加入时，Aapt可以与其结合并产生荧光。ACE不引起比色反应，TMX不引起荧光。同时采用比色法和荧光检测方法，可以实现对两种目标的无干扰检测。

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来源期刊

Journal of Food Composition and Analysis 工程技术-食品科技

CiteScore

6.20

自引率

11.60%

发文量

601

审稿时长

53 days

期刊介绍： The Journal of Food Composition and Analysis publishes manuscripts on scientific aspects of data on the chemical composition of human foods, with particular emphasis on actual data on composition of foods; analytical methods; studies on the manipulation, storage, distribution and use of food composition data; and studies on the statistics, use and distribution of such data and data systems. The Journal''s basis is nutrient composition, with increasing emphasis on bioactive non-nutrient and anti-nutrient components. Papers must provide sufficient description of the food samples, analytical methods, quality control procedures and statistical treatments of the data to permit the end users of the food composition data to evaluate the appropriateness of such data in their projects. The Journal does not publish papers on: microbiological compounds; sensory quality; aromatics/volatiles in food and wine; essential oils; organoleptic characteristics of food; physical properties; or clinical papers and pharmacology-related papers.