Exogenous hydroxyapatite and silicon nanoparticles mitigate salinity and lead (Pb) stress in coneflower (Echinacea angustifolia) by adjusting growth and biochemical attributes

Abstract

Heavy metals and salinity represent two of the most eminent challenges facing agriculture. Recently, there has been a lot of interest in silicon nanoparticles (Si NPs) and hydroxyapatite nanoparticles (HAP NPs) for managing abiotic stress. Their comparative application in salinity and lead (Pb) stress, however, has not received much attention. Therefore, the current study was carried out to determine the effects of HAP and Si NPs on mitigating Pb and salinity stress in coneflower (Echinacea angustifolia) through physiological and biochemical attributes. The plants were sprayed with HAP and Si NPs at 200 mg L⁻¹ and exposed to Pb (600 mg kg⁻¹ of soil) and salinity (100 mM NaCl). The results showed that Pb and salinity respectively lowered shoot weight (29 % and 36 %), root weight (20 % and 23 %), chlorophyll (Chl) a+b (31 % and 35 %), relative water content (RWC, 16 % and 19 %), total phenolic content (TPC, 5 % and 14 %), total flavonoid content (TFC, 11 % and 25 %), essential oil (EO) content (14 % and 26 %), EO yield (39 % and 52 %), but increased malondialdehyde (MDA, 43 % and 66 %) and proline (11 % and 18 %) in plants without NPs application. The use of Si and HAP NPs modulated Pb and salinity as their combination increased shoot weight (30 %), root weight (13 %), Chl a+b (17 %), RWC (15 %), proline (40 %), TPC (17 %), TFC (37 %), EO content (29 %), EO yield (68 %), but lowered MDA (24 %) in salt-exposed plants. The heat map showed that EO yield exhibited the greatest variability among the traits. The use of combined HAP and Si NPs suggests the potential of this combined treatment to mitigate salinity and Pb stress.