Construction of high-yielding edeine strains and an initial exploration of their control efficacy against crop pathogens

IF 5.2 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Chemical and Biological Technologies in Agriculture Pub Date : 2025-05-21 DOI:10.1186/s40538-025-00786-y

Liang Zhang, Ziyue Chen, Fei Xia, Tianbo Liu, Qingshu Liu, Wu Chen

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引用次数: 0

Abstract

Background

Edeine, a non-ribosomal antibiotic produced by Brevibacillus brevis X23, has a broad-spectrum antimicrobial activity against plant pathogens, but its low yield in wild-type strains limits its potential for agricultural applications. This study aimed to enhance edeine production by genetically engineering B. brevis X23.

Methods

Red/ET homologous recombination technology was used to construct engineered strain X23(ΔabrB)::P_mwp by knocking out global negative regulator AbrB and replacing the natural promoter of the edeine biosynthesis gene cluster (ede BGC) with the strong P_mwp promoter.

Results

Quantitative PCR revealed significantly increased ede BGC transcription levels in X23(ΔabrB)::P_mwp compared to the wild-type strain. High-performance liquid chromatography–mass spectrometry (HPLC–MS) demonstrated a 10.1-fold increase in the edeine peak area with the final yield reaching 97.3 mg/L. In pot experiments for tobacco bacterial wilt (pathogen name Ralstonia solanacearum) control, X23(ΔabrB)::P_mwp showed an efficacy of 82.9%, representing a 32.6% improvement over the wild-type strain (62.5%). The engineered strain also demonstrated an increased plate inhibition capacity of 20.5–60.9% against Verticillium dahliae Kleb, Rhizoctonia solani, and Fusarium oxysporum in cotton, indicating its potential application in crop protection.

Conclusions

Therefore, this study yielded an engineered strain with increased edeine production and enhanced biocontrol efficacy, contributing to the development of biological control methods for plant diseases.

Graphical Abstract

查看原文本刊更多论文

edeine高产菌株的构建及其对作物病原菌防治效果的初步探索

dedeine是由短芽孢杆菌X23生产的一种非核糖体抗生素，对植物病原体具有广谱抗菌活性，但其野生型菌株产量低，限制了其在农业上的应用潜力。本研究旨在利用基因工程技术提高短芽孢杆菌X23的产量。方法采用red /ET同源重组技术，敲除edeine生物合成基因簇（ede BGC）的天然启动子，并用强启动子替换edeine生物合成基因簇（ede BGC），构建工程菌株X23（ΔabrB）：：Pmwp。结果定量PCR结果显示，X23（ΔabrB）：：Pmwp中ede BGC转录水平较野生型明显升高。高效液相色谱-质谱（HPLC-MS）分析结果表明，该方法的苯胺峰面积增加了10.1倍，最终产率达到97.3 mg/L。盆栽试验中，X23（ΔabrB）：：Pmwp对烟草青枯病（Ralstonia solanacearum）的防治效果为82.9%，比野生型（62.5%）提高32.6%。该基因工程菌株对棉花黄萎病菌、枯丝核菌和尖孢镰刀菌的平板抑制能力提高20.5 ~ 60.9%，在作物保护方面具有一定的应用前景。结论本研究获得的工程菌株具有较高的乙胺产量和较强的生物防治效果，为植物病害生物防治方法的发展奠定了基础。图形抽象

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemical and Biological Technologies in Agriculture Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

6.80

自引率

3.00%

发文量

审稿时长

15 weeks

期刊介绍： Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture. This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population. Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.