{"title":"Projection-specific and reversible functional blockage in the association cortex of macaque monkeys","authors":"Seiichirou Yokoo , Takayasu Higo , Florian Gerard-Mercier , Mineki Oguchi , Masamichi Sakagami , Haruhiko Bito , Masayuki Sakamoto , Noritake Ichinohe , Keiji Tanaka","doi":"10.1016/j.neures.2025.05.004","DOIUrl":null,"url":null,"abstract":"<div><div>The functional manipulation techniques based on optogenetics have been widely and effectively utilized in the rodent brain. However, the applications of these techniques to the macaque cerebral cortex, particularly those to the prefrontal cortex, have been limited due to the extensive size and complex functional organization of each prefrontal area. In this study, we developed projection-specific and reversible functional blockade methods applicable to areas of the macaque prefrontal cortex, based on chemogenetic techniques. In chemogenetics, once a pair of viral vectors has been injected into the regions of projection source and target, the projection-specific functional blockage can be initiated through the oral, intravenous, or intramuscular administration of an appropriate pharmaceutical agent. Two methods were developed using two different effector proteins, an inhibitory DREADD, hM4Di, and tetanus toxin, given the substantial discrepancy in the on-off time course of functional blockade between the two. The Cre-DIO system was combined with hM4Di, and the Tet-On system with tetanus toxin. The effectiveness of these methods was evaluated by developing an electrophysiological assay using photic stimulation and field potential recordings.</div></div>","PeriodicalId":19146,"journal":{"name":"Neuroscience Research","volume":"217 ","pages":"Article 104909"},"PeriodicalIF":2.4000,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neuroscience Research","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0168010225000860","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
The functional manipulation techniques based on optogenetics have been widely and effectively utilized in the rodent brain. However, the applications of these techniques to the macaque cerebral cortex, particularly those to the prefrontal cortex, have been limited due to the extensive size and complex functional organization of each prefrontal area. In this study, we developed projection-specific and reversible functional blockade methods applicable to areas of the macaque prefrontal cortex, based on chemogenetic techniques. In chemogenetics, once a pair of viral vectors has been injected into the regions of projection source and target, the projection-specific functional blockage can be initiated through the oral, intravenous, or intramuscular administration of an appropriate pharmaceutical agent. Two methods were developed using two different effector proteins, an inhibitory DREADD, hM4Di, and tetanus toxin, given the substantial discrepancy in the on-off time course of functional blockade between the two. The Cre-DIO system was combined with hM4Di, and the Tet-On system with tetanus toxin. The effectiveness of these methods was evaluated by developing an electrophysiological assay using photic stimulation and field potential recordings.
期刊介绍:
The international journal publishing original full-length research articles, short communications, technical notes, and reviews on all aspects of neuroscience
Neuroscience Research is an international journal for high quality articles in all branches of neuroscience, from the molecular to the behavioral levels. The journal is published in collaboration with the Japan Neuroscience Society and is open to all contributors in the world.