Production of MLM-type structured lipids from avocado oil catalyzed by immobilized lipase on corn cob powder: Assessment of antiproliferative potential on Hep-G2 cell line

IF 4.8 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience Pub Date : 2025-05-14 DOI:10.1016/j.fbio.2025.106824

Maryli Jessenia Bohorquez-Peña , Brandon Vargas-Suaza , Kevin Esteban Garzón-Alonso , Julio Cesar Rendón-Londoño , Yuly Nataly Franco-Tobón , Claudia Lorena Gómez-Herrera , Mónica Mesa , Julián Paul Martínez-Galán

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引用次数: 0

Abstract

The demand for natural and healthy products has driven research into new value-added products derived from avocado (Persea Americana var. Hass) oil. For example, the exploration of the production of structured lipids (SL), which are modified triacylglycerides with enhanced nutritional and functional properties. Enzymatic engineering of structured lipids using immobilized lipases allows for greater economy and stability under reaction conditions. In this study, medium- and long-chain structured lipids were produced from avocado oil caprylic acid by enzymatic acidolysis. Lipase from Thermomyces lanuginosus was immobilized on corn cob powder. Efficient incorporation of caprylic acid (C8:0) into avocado oil was achieved with a maximum of 31.11 % at sn-1 or sn-3 positions. However, incorporation did not occur simultaneously into triacylglycerides, and lipase reusability of up to 4 cycles. In the hepatocarcinoma cell line Hep-G2, the structured and hydrolyzed lipids inhibited cell viability, suggesting their potential for future studies related to the maintenance of liver health.

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玉米芯粉固定化脂肪酶催化牛油果油制备mlm型结构脂质：对Hep-G2细胞株抗增殖潜能的评价

对天然和健康产品的需求推动了对鳄梨（Persea Americana var. Hass）油衍生的新增值产品的研究。例如，探索结构脂类（SL）的生产，这是一种具有增强营养和功能特性的改性甘油三酯。利用固定化脂肪酶进行结构脂质的酶工程，在反应条件下具有更大的经济性和稳定性。本研究以鳄梨油辛酸为原料，采用酶解法制备了中、长链结构脂质。以玉米棒子粉为载体，固定化了褐豆热酵素脂肪酶。在sn-1或sn-3位置，辛酸（C8:0）在鳄梨油中的有效掺入率最高为31.11%。然而，并没有同时掺入到甘油三酯中，并且脂肪酶可重复使用达4个循环。在肝癌细胞系Hep-G2中，结构脂质和水解脂质抑制细胞活力，这表明它们在未来与肝脏健康维持相关的研究中具有潜力。

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来源期刊

Food Bioscience Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

6.40

自引率

5.80%

发文量

671

审稿时长

27 days

期刊介绍： Food Bioscience is a peer-reviewed journal that aims to provide a forum for recent developments in the field of bio-related food research. The journal focuses on both fundamental and applied research worldwide, with special attention to ethnic and cultural aspects of food bioresearch.