Lactobacillus plantarum in high methoxylated pectin-based encapsulation systems supports gut epithelial wound healing in vitro

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health Pub Date : 2025-05-13 DOI:10.1016/j.fhfh.2025.100218

Felipe Galvez-Jiron , Lucas de Freitas Pedrosa , Luis Silva Lagos , Xin Tang , Traudy Wandersleben , Rodrigo Navia , Stephan Drusch , Francisca Acevedo , Paul de Vos

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Abstract

Wound healing and gut epithelial barrier regulation are crucial for intestinal homeostasis. Effective repair of gut epithelial wounds is crucial for re-establishing the mucosal barrier and resolving inflammation. Pectins, versatile polysaccharides in food, protect sensitive components, such as living bacteria, during gastrointestinal transit and support wound healing by promoting cell proliferation and migration.

This study investigated the effects of encapsulated Lactobacillus plantarum WCFS1 on cell proliferation and migration during epithelial wound healing, using pectin-based beads stabilized with alginate or chitosan. Pectin from lemon, with two degrees of methyl esterification (DM18 and DM88), produced four microbead types. T84 cells were incubated with these beads for 24 hours. The results revealed that cell proliferation and migration were influenced by the bead type, the degree of methyl esterification of the pectin, and the inclusion of L. plantarum. Empty DM88 pectin beads improved cell proliferation while reducing migration tendencies, whereas DM18 beads delayed migration.Beads containing L. plantarum demonstrated different effects: DM18 beads enhanced proliferation without inhibiting migration, while DM88 beads reduced migration. Both empty and L. plantarum-loaded beads decreased CCL20 production. Alginate/pectin beads with L. plantarum increased IL-8, while chitosan/pectin beads delayed migration, reduced CCL20 secretion, and modulated IL-8 levels.

In conclusion, DM88 pectin beads without L. plantarum and DM18 beads containing L. plantarum supported cell proliferation over migration during epithelial wound healing. These findings suggest that pectin-based encapsulation systems may play a role in promoting gut health, protecting barrier integrity, and delivering therapeutic agents.

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植物乳杆菌在高甲氧基果胶为基础的包封系统支持肠道上皮伤口愈合体外

伤口愈合和肠上皮屏障调节对肠道内稳态至关重要。肠道上皮伤口的有效修复对于重建粘膜屏障和消除炎症至关重要。果胶是食物中的多功能多糖，在胃肠道运输过程中保护敏感成分，如活细菌，并通过促进细胞增殖和迁移来支持伤口愈合。本研究利用海藻酸盐或壳聚糖稳定的果胶微球，研究了包封植物乳杆菌WCFS1对上皮伤口愈合过程中细胞增殖和迁移的影响。柠檬果胶经DM18和DM88两种程度的甲基酯化反应，可产生四种微珠。用这些珠培养T84细胞24小时。结果表明，细胞的增殖和迁移受珠粒类型、果胶的甲基酯化程度和植物乳杆菌包合物的影响。空DM88胶珠促进细胞增殖，降低迁移倾向，DM18胶珠则延迟迁移。含有植物乳杆菌的微球表现出不同的效果：DM18微球促进了植物乳杆菌的增殖，但不抑制迁移，DM88微球则抑制了迁移。空珠和载L. plantaram珠均降低CCL20产量。海藻酸盐/果胶珠可提高植物乳杆菌的IL-8水平，壳聚糖/果胶珠可延缓迁移，减少CCL20分泌，调节IL-8水平。综上所述，不含植物乳杆菌的DM88果胶珠和含有植物乳杆菌的DM18果胶珠在上皮伤口愈合过程中支持细胞增殖和迁移。这些发现表明，基于果胶的包封系统可能在促进肠道健康、保护屏障完整性和递送治疗剂方面发挥作用。

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