Distribution of haploid chromosomes into separate nuclei in two pathogenic fungi.

IF 44.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Pub Date : 2025-05-15 DOI:10.1126/science.abn7811

Yan Xu, Lei Tian, Jinyi Tan, Weijie Huang, Josh Li, Nigel O'Neil, Martin Hirst, Phil Hieter, Yuelin Zhang, Xin Li

引用次数: 0

Abstract

Nuclei define eukaryotes, enabling macromolecular compartmentalization and cellular regulation. Each nucleus is believed to contain one or more haploid sets of chromosomes (1N). However, we discovered that haploid cells of the pathogenic fungi Sclerotinia sclerotiorum and Botrytis cinerea distribute their chromosomes such that each of their nuclei contains only a subset of the haploid chromosomes (≤½N). The unusual chromosomal distribution was confirmed by cellular and molecular methods including chromosome counting, fluorescence in situ hybridization, flow cytometry-based DNA measurements, and single-nucleus polymerase chain reaction experiments. This phenomenon challenges fundamental assumptions about nuclear organization and opens fresh avenues in chromosome biology.

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两种致病真菌中单倍体染色体在分离细胞核中的分布。

细胞核定义真核生物，使大分子区隔化和细胞调节成为可能。每个细胞核被认为包含一个或多个单倍体染色体组（1N）。然而，我们发现病原真菌菌核菌（Sclerotinia sclerotiorum）和灰霉病菌（Botrytis cinerea）的单倍体细胞的染色体分布使得它们的每个细胞核只包含单倍体染色体的一个子集（≤½N）。通过细胞和分子方法，包括染色体计数、荧光原位杂交、基于流式细胞术的DNA测量和单核聚合酶链反应实验，证实了异常的染色体分布。这一现象挑战了关于核组织的基本假设，并为染色体生物学开辟了新的途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Science 综合性期刊-综合性期刊

CiteScore

61.10

自引率

0.90%

发文量

审稿时长

2.1 months

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