Different Forms of Manganese Provide Distinctive Metabolomics Signatures and Bioactive Profiles in Artemisia annua.

IF 3 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Phytochemical Analysis Pub Date : 2025-05-16 DOI:10.1002/pca.3538

Hajar Salehi, Abdolkarim Chehregani Rad, Luigi Lucini

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引用次数: 0

Abstract

Introduction: Natural products are among the main ingredients of medicinal plants, and strategies that enhance their bioactive profile by elemental supplementation have emerged recently. Manganese is involved in various plant secondary metabolism pathways among different micronutrients.

Objectives: This study investigated the effects of ionic (MnSO₄, MnCl₂), bulk (Mn₂O₃), and nano (Mn₂O₃-NP) manganese forms on Artemisia annua's secondary metabolism. It also studied the influence of the application method (seed priming vs. seed priming + foliar).

Methods: For this purpose, untargeted UHPLC-QTOF-HRMS metabolomics was conducted.

Results: The findings revealed that Mn form and application method significantly influenced the metabolomic profile and secondary metabolite composition of the leaves and inflorescences, regardless of tissue type. Metabolomic profiling using untargeted analysis and multivariate statistical tools (PCA, PLS-DA, and VIP scoring) showed significant variation in bioactive compound accumulation. Mn₂O₃ and MnCl₂ were most effective in enhancing nitrogen-containing compounds, phenylpropanoids, flavonoids, and terpenoids, possibly via ROS-mediated biosynthesis. Mn₂O₃ strongly increased lignans, while Mn₂O₃-NP showed the highest artemisinin accumulation (3.2-3.7 mg g^-1 FW) compared to MnCl₂ and Mn₂O₃ (0.2-1.7 mg g^-1 FW). Key pharmacological metabolites such as vincristine, Momilactone A, and terbinafine were identified by VIP2 analysis.

Conclusion: Mn₂O₃-NP application through seed priming is a promising and cost-effective approach to modulate bioactive metabolite production in Artemisia annua.

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不同形式的锰在黄花蒿中提供不同的代谢组学特征和生物活性谱。

天然产物是药用植物的主要成分之一，近年来通过补充元素来提高其生物活性的策略已经出现。锰参与植物不同微量元素间的多种次生代谢途径。目的：研究离子锰（MnSO₄、MnCl₂）、大块锰（Mn₂O₃）和纳米锰（Mn₂O₃-NP）形式对黄花蒿次生代谢的影响。研究了灌种方式与灌种+叶面灌种方式的影响。方法：采用非靶向UHPLC-QTOF-HRMS代谢组学方法。结果：研究结果表明，无论组织类型如何，Mn形态和施用方式均显著影响叶片和花序的代谢组学特征和次生代谢物组成。使用非靶向分析和多变量统计工具（PCA、PLS-DA和VIP评分）的代谢组学分析显示，生物活性化合物积累存在显著差异。Mn₂O₃和MnCl₂对含氮化合物、苯丙烷、黄酮类化合物和萜类化合物最有效，可能是通过ros介导的生物合成。Mn₂O₃增加了木脂素，而Mn₂O₃-NP比MnCl₂和Mn₂O₃（0.2 ~ 1.7 mg g-1 FW）积累了最多的青蒿素（3.2 ~ 3.7 mg g-1 FW）。通过VIP2分析鉴定出长春新碱、莫内通A、特比萘芬等关键药理代谢物。结论：通过种子启动应用Mn₂O₃-NP调控黄花蒿生物活性代谢物的产生是一种有前景且经济的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Phytochemical Analysis 生物-分析化学

CiteScore

6.00

自引率

6.10%

发文量

审稿时长

1.7 months

期刊介绍： Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.