An UHPLC-MS/MS method for the determination of antiparasitic drugs, including avermectins, in avian eggs, featuring a novel standard addition approach to validation.

IF 2.3 3区农林科学 Q2 CHEMISTRY, APPLIED

Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment Pub Date : 2025-05-14 DOI:10.1080/19440049.2025.2503364

Philip Mullen, Cheryl Rainey, Matthew Walker, Anna Gadaj

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Abstract

Antiparasitic drugs have widespread use in livestock to control parasitic infections. Their use can lead to residues in animal-derived food products, which can pose potential health risks to consumers. Therefore, a method has been developed for the semi-quantitative screening of 51 antiparasitic drug residues in avian eggs, using a QuEChERS based extraction method and analysis by UHPLC-MS/MS. This extension to coverage of these analytes also includes an approach to the analysis of the traditionally difficult avermectin class of analytes using source optimisation and post-column infusion. The method was validated according to CIR (EU) 2021/808 and the detection capability (CCβ) was set at 5 µg kg^-1 for all analytes, except fipronil and its metabolites, at 1.25 µg kg^-1. Additionally, a novel standard addition based confirmatory method for 10 analytes with MRL values was implemented, in accordance with EURL guidance.

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采用高效液相色谱-质谱联用（UHPLC-MS/MS）方法测定禽蛋中抗寄生虫药物阿维菌素的含量。

抗寄生虫药物在家畜中广泛使用以控制寄生虫感染。它们的使用可能导致动物源性食品中残留，这可能对消费者构成潜在的健康风险。为此，建立了一种基于QuEChERS提取方法和UHPLC-MS/MS分析方法对禽蛋中51种抗寄生虫药物残留进行半定量筛选的方法。对这些分析物的覆盖范围的扩展还包括使用源优化和柱后输注分析传统上困难的阿维菌素类分析物的方法。该方法按照CIR (EU) 2021/808进行验证，除氟虫腈及其代谢物为1.25µg kg-1外，所有分析物的检测能力（CCβ）为5µg kg-1。此外，根据EURL指南，对10种具有MRL值的分析物实施了一种新的基于标准添加的验证方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment CHEMISTRY, APPLIED-FOOD SCIENCE & TECHNOLOGY

CiteScore

7.40

自引率

6.90%

发文量

136

审稿时长

3 months

期刊介绍： Food Additives & Contaminants: Part A publishes original research papers and critical reviews covering analytical methodology, occurrence, persistence, safety evaluation, detoxification and regulatory control of natural and man-made additives and contaminants in the food and animal feed chain. Papers are published in the areas of food additives including flavourings, pesticide and veterinary drug residues, environmental contaminants, plant toxins, mycotoxins, marine biotoxins, trace elements, migration from food packaging, food process contaminants, adulteration, authenticity and allergenicity of foods. Papers are published on animal feed where residues and contaminants can give rise to food safety concerns. Contributions cover chemistry, biochemistry and bioavailability of these substances, factors affecting levels during production, processing, packaging and storage; the development of novel foods and processes; exposure and risk assessment.