Regulation of proanthocyanidin accumulation by VvMYB1 in response to vine water constraint in 'Marselan' grape berries

IF 3.9 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2025-05-01 DOI:10.1016/j.scienta.2025.114184

Xiaobin Xue , Chenyang Hou , Pengcheng Yang , Hongyuan Hu , Linxin Li , Jinyang Li , Hongjun Liu , Yu Zhou , Lin Ning , Dongmei Li , Zhenping Wang

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引用次数: 0

Abstract

Proanthocyanidin (PA) is primary flavonoid compound in grape berries and red wines, helping plants resist various abiotic stresses. However, the specific role of PA in response to water constraints has not been extensively studied. In this study, six-year-old field grown 'Marselan' (Vitis vinifera L.) grapevines were subjected to different levels of water constraint in 2022, including well-irrigated (CK) and moderate water constraint (MW) treatments. The main objective was to evaluate the impact of vine water status on PA metabolism and investigate the underlying mechanism. The results indicated that MW treatment not only improved grape berries quality but also significantly increased the content of proanthocyanidin B1 (PB1), PB2, PB3, and PC1. Moreover, transcriptome sequencing and qRT-PCR analysis revealed that MW treatment led to higher expression levels of VvLAR2 and VvMYB1 at the pre-berry development compared to other stages. Furthermore, yeast one-hybrid (Y1H) and dual-luciferase reporter assays revealed that VvMYB1 directly bound and activated the promoter (CAACTG site) of VvLAR2. Finally, overexpression of the VvMYB1 gene in grape berries and callus resulted in enhanced PA accumulation. Meanwhile, the expression level of VvLAR2 was also increased. The above results suggest that VvMYB1 positively controls PA accumulation. This study provides a new understanding of the connection between PA biosynthesis and vine water status.

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vmyb1对‘Marselan’葡萄果实水分胁迫下原花青素积累的调控

原花青素（PA）是葡萄果实和红酒中的主要类黄酮化合物，帮助植物抵抗各种非生物胁迫。然而，PA在应对水分限制方面的具体作用尚未得到广泛研究。在本研究中，在2022年对6年大田种植的“Marselan”（Vitis vinifera L.）葡萄进行了不同程度的水分限制，包括良好灌溉（CK）和适度水分限制（MW）处理。主要目的是评估葡萄水分状况对PA代谢的影响，并探讨其潜在机制。结果表明，MW处理不仅改善了葡萄果实品质，而且显著提高了原花青素B1 （PB1）、PB2、PB3和PC1的含量。此外，转录组测序和qRT-PCR分析显示，MW处理导致VvLAR2和VvMYB1在浆果发育前的表达水平高于其他阶段。此外，酵母单杂交（Y1H）和双荧光素酶报告基因检测显示，VvMYB1直接结合并激活了VvLAR2的启动子（CAACTG位点）。最后，VvMYB1基因在葡萄果实和愈伤组织中的过表达导致PA积累增加。同时，VvLAR2的表达水平也有所升高。以上结果提示，VvMYB1对PA积累具有正向控制作用。本研究为PA生物合成与葡萄水分状况的关系提供了新的认识。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.