Identification of a novel calcium transport-promoting peptide from phosvitin Hydrolysate: Establishment of a Caco-2 cell monolayer, sequence determination, and molecular interaction analysis

IF 4.8 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience Pub Date : 2025-05-13 DOI:10.1016/j.fbio.2025.106825

Wei Liu , Songming Li , Zhaoxia Cai , Yi Wang , Meiyu Liu , Xi Huang

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引用次数: 0

Abstract

This research aimed to purify and identify calcium transport-promoting peptides from phosvitin hydrolysate (PPPs) using anion-exchange chromatography, Caco-2 cell monolayer assays, determining peptide sequence, and molecular docking. Four fractions were obtained from PPPs using anion-exchange chromatography. The third fraction (PPP3) exhibited the highest calcium chelating capacity (98.10 ± 0.92 %) at a peptide to calcium mass ratio of 6 to 1. Based on the Caco-2 cell monolayers, PPP3 exhibited the highest calcium transport-promoting activity compared to PPPs, which might be associated with the transient receptor potential vanilloid 6 (TRPV6) channel. The sequences of peptides were identified from PPP3 using chromatography-tandem mass spectrometry. Eight peptides with <1 kDa molecular weights and <10 amino acids were docked to TRPV6 protein. Among them, LSKIWGR, a water-soluble peptide, showed the highest binding affinity with TRPV6 through hydrogen bonds and hydrophobic interactions, which was −8.4 kcal/mol. Compared to the PPP3 component, LSKIWGR showed a stronger enhancement of calcium transport (10.55 ± 0.10 μg/well) on the Caco-2 cell monolayer. In summary, this study isolated a novel peptide (LSKIWGR) from PPPs that promotes calcium transport via the TRPV6 channel. It provides a theoretical basis for further investigation of its calcium transport-promoting activity in vivo.

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从光维素水解物中鉴定一种新的钙转运促进肽：Caco-2细胞单层的建立、序列测定和分子相互作用分析

本研究旨在通过阴离子交换色谱法、Caco-2细胞单层法、确定肽序列和分子对接，从光维素水解物（PPPs）中纯化和鉴定钙转运促进肽。用阴离子交换色谱法从PPPs中分离得到4个组分。第三部分PPP3在肽钙质量比为6:1时，螯合钙的能力最高（98.10±0.92%）。基于ccao -2细胞单层，PPP3比PPPs表现出最高的钙转运促进活性，这可能与瞬时受体电位香草样蛋白6 （TRPV6）通道有关。利用层析-串联质谱法从PPP3中鉴定肽序列。8个分子量为1kda的肽和10个氨基酸与TRPV6蛋白对接。其中，水溶性肽LSKIWGR通过氢键和疏水相互作用与TRPV6的结合亲和力最高，为−8.4 kcal/mol。与PPP3组分相比，LSKIWGR对Caco-2细胞单层钙转运的促进作用更强（10.55±0.10 μg/孔）。总之，本研究从PPPs中分离出一种促进钙通过TRPV6通道运输的新肽（LSKIWGR）。为进一步研究其在体内促进钙转运的活性提供了理论基础。

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来源期刊

Food Bioscience Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

6.40

自引率

5.80%

发文量

671

审稿时长

27 days

期刊介绍： Food Bioscience is a peer-reviewed journal that aims to provide a forum for recent developments in the field of bio-related food research. The journal focuses on both fundamental and applied research worldwide, with special attention to ethnic and cultural aspects of food bioresearch.