Novel bispecific monoclonal antibody-based dual-analyte immunochromatographic strip for the detection of aflatoxin B1 and ochratoxin A in cereal samples

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-05-17 DOI:10.1016/j.foodchem.2025.144762

Shih-Wei Wu , Wei Hong Tai , Chia-Yu Hsieh , Biing-Hui Liu , Feng-Yih Yu

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Abstract

We have developed a novel bispecific monoclonal antibody (mAb) for the simultaneous detection of aflatoxin B1 (AFB1) and ochratoxin A (OTA). The specific mAb was used to establish a sensitive competitive direct ELISA (cdELISA) and dual-analyte immunochromatographic strip (immunostrip). The cdELISA achieved IC₅₀ values of 0.06 ng/mL for AFB1 and 1.02 ng/mL for OTA, with limits of detection (LOD) of 0.004 ng/mL and 0.24 ng/mL, respectively. The dual-analyte immunostrip exhibited visual detection limits of 0.5 ng/mL for AFB1 and 5 ng/mL for OTA. Recovery rates for spiked cereal samples ranged from 91 % to 110 % for AFB1 and from 99 % to 107 % for OTA. The analysis of 15 cereal samples using the cdELISA detected AFB1 and OTA contamination consistent with HPLC analysis, validating the reliability of both methods. This bispecific mAb offers a rapid and efficient approach for monitoring AFB1 and OTA in food, enhancing food safety efforts.

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基于双特异性单克隆抗体的双分析物免疫层析试纸条检测谷物样品中黄曲霉毒素B1和赭曲霉毒素A

我们开发了一种新的双特异性单克隆抗体（mAb），用于同时检测黄曲霉毒素B1 （AFB1）和赭曲霉毒素a （OTA）。利用特异性单抗建立灵敏的竞争性直接ELISA （cdELISA）和双分析物免疫层析条带（immunostrip）。cdELISA检测AFB1的IC50值为0.06 ng/mL， OTA的IC50值为1.02 ng/mL，检出限（LOD）分别为0.004 ng/mL和0.24 ng/mL。双分析免疫条对AFB1和OTA的视觉检出限分别为0.5 ng/mL和5 ng/mL。加标谷物样品中AFB1的回收率为91 % ~ 110 %，OTA的回收率为99 % ~ 107 %。对15份谷物样品进行cdELISA分析，发现AFB1和OTA污染与HPLC分析结果一致，验证了两种方法的可靠性。这种双特异性单抗为监测食品中AFB1和OTA提供了快速有效的方法，加强了食品安全工作。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.