Micronucleus and Nuclear Budding Help to Identify Malignancy in Thyroid Fine Needle Aspiration Cytology.

IF 1.1 4区医学 Q4 CELL BIOLOGY

Cytopathology Pub Date : 2025-04-25 DOI:10.1111/cyt.13505

Nur Gizem Kocaoğlu Çelik, Ayşegül Aksoy Altınboğa, Tuba Dilay Kökenek Ünal

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引用次数: 0

Abstract

Objective: Micronucleus (MN) and Nuclear Budding (NB) Have Been Used as an Important Stimulus for Nuclear Atypia in Revealing Chromosomal Damage and Cancer Risk. This Study Aims to Assess MN and NB as Nuclear Parameters and Explore Their Role in Differential Diagnosis of Thyroid FNA and "Bethesda System for Reporting Thyroid Cytology" (BSRTC).

Methods: The Study Included 350 Thyroid FNA Samples Categorised According to BSRTC. NB And MN Were Evaluated in 1000 Thyrocytes on FNA Slides. The Thyroidectomy Sections Were Divided Into Three Categories as 'Malignant', 'Low-Risk' and 'Benign'.

Results: A Total of 350 Cases Were Classified as follows: 55 (15.71%) Benign, 159 (45.43%) Atypia of Undetermined Significance (AUS), 20 (5.71%) Follicular Neoplasm (FN), 48 (13.71%) Suspicious for Malignancy (SFM) and 68 (19.43%) Malignant. In comparison to all other cytopathologic diagnostic groups, the benign group had significantly lower levels of MN and NB (p < 0.001). The AUS group's MN and NB counts were significantly lower than those of the FN, SFM and malignant groups (MN for AUS-FN/SFM/M p < 0.001, NB for AUS-FN p = 0.01, NB for AUS-SFM/M p < 0.001, respectively). MN and NB counts were greater in the AUS group for cases with a malignant histopathologic diagnosis than for those with a benign histopathologic diagnosis (p < 0.001). MN and NB cut-off values > 5.50 and > 14.50, respectively, suggest high sensitivity and specificity of malignancy.

Conclusions: Increased Frequency of MN and NB closely associated with an increased risk of malignancy in thyroid FNA. Assessing MN and NB in thyroid FNA may help better stratify the risk in the AUS category.

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微核和核出芽有助于甲状腺细针穿刺细胞学鉴别恶性肿瘤。

目的：微核（MN）和核芽殖（NB）作为核异型性的重要刺激因子，揭示染色体损伤和肿瘤风险。本研究旨在评估MN和NB作为核参数，并探讨其在甲状腺FNA和“Bethesda甲状腺细胞学报告系统”（BSRTC）鉴别诊断中的作用。方法：采用按BSRTC分类的甲状腺FNA样本350例。在FNA载玻片上测定1000个甲状腺细胞的NB和MN。甲状腺切除术分为“恶性”、“低风险”和“良性”三大类。结果：共350例，其中良性55例（15.71%），异型不明（AUS） 159例（45.43%），滤泡性肿瘤20例（5.71%），可疑恶性48例（13.71%），恶性68例（19.43%）。与所有其他细胞病理学诊断组相比，良性组的MN和NB水平显著降低（p分别为5.50和14.50），提示恶性肿瘤具有较高的敏感性和特异性。结论：甲状腺FNA中MN和NB频率的增加与恶性肿瘤风险的增加密切相关。评估甲状腺FNA中的MN和NB可能有助于更好地划分AUS类别的风险。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cytopathology 生物-病理学

CiteScore

2.30

自引率

15.40%

发文量

107

审稿时长

6-12 weeks

期刊介绍： The aim of Cytopathology is to publish articles relating to those aspects of cytology which will increase our knowledge and understanding of the aetiology, diagnosis and management of human disease. It contains original articles and critical reviews on all aspects of clinical cytology in its broadest sense, including: gynaecological and non-gynaecological cytology; fine needle aspiration and screening strategy. Cytopathology welcomes papers and articles on: ultrastructural, histochemical and immunocytochemical studies of the cell; quantitative cytology and DNA hybridization as applied to cytological material.