Functional analysis of AcARF18 in kiwifruit resistance to Botrytis cinerea.

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-05-07 DOI:10.1002/jsfa.14345

Zhexin Li, Yijia Ma, Yuan Sui, Jiaqi Yang, Shuai Yang, Run Xiong, Guohua Liu, Yusong Jiang

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引用次数: 0

Abstract

Background: Investigating the role of AcARF18 in resistance to Botrytis cinerea in kiwifruit (Actinidia chinensis) may enrich our understanding of the disease resistance regulatory mechanisms and provide genetic resource reserves for disease resistance molecular breeding in kiwifruit.

Results: Virus-induced gene silencing was used to investigate the functional role of AcARF18 in kiwifruit against B. cinerea. Silencing of AcARF18 (AcARF18-TRV) enhanced the resistance of kiwifruit to B. cinerea, suggesting that AcARF18 might negatively regulate kiwifruit resistance to the fungal pathogen. Physiological index analysis showed that the activity of defense enzymes, and contents of total phenols and flavonoids, and the content of indole-3-acetic acid and salicylic acid were higher in AcARF18-TRV kiwifruit than that in the control group (CK) with or without B. cinerea. RNA-seq analysis with a log₂ fold change of ≥1 and false discovery rate of <0.01 revealed 2184 differentially expressed genes (DEGs) in AcARF18-TRV versus CK comparison. Of these DEGs, 1130 were upregulated and 1054 were downregulated in the silenced group. The majority of the selected DEGs were enriched in 'secondary metabolite synthesis' and 'plant hormone signal transduction' pathways.

Conclusion: Our results reveal the molecular mechanism by which AcARF18 regulates kiwifruit resistance to B. cinerea, and further provide genetic resources for the molecular breeding of kiwifruit to enhance its disease resistance. © 2025 Society of Chemical Industry.

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AcARF18在猕猴桃抗灰霉病中的功能分析。

背景：研究AcARF18在猕猴桃（Actinidia chinensis）抗病过程中的作用，可以丰富我们对猕猴桃抗病调控机制的认识，为猕猴桃抗病分子育种提供遗传资源储备。结果：采用病毒诱导基因沉默的方法研究了AcARF18在猕猴桃中对灰绿杆菌的功能作用。AcARF18 （AcARF18- trv）的沉默增强了猕猴桃对B. cinerea的抗性，提示AcARF18可能负向调节猕猴桃对该真菌病原菌的抗性。生理指标分析表明，AcARF18-TRV猕猴桃防御酶活性、总酚和总黄酮含量、吲哚-3-乙酸和水杨酸含量均高于对照（CK）。结论：我们的研究结果揭示了AcARF18调控猕猴桃抗病的分子机制，为猕猴桃抗病的分子育种提供遗传资源，进一步提高猕猴桃抗病能力。©2025化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.