Metabolomics and network pharmacology reveal the anti-inflammatory core substances and mechanisms of a new cultivar of high-polyphenol lettuce.

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-04-29 DOI:10.1002/jsfa.14270

Qianshan Lu, Wenxuan Tian, Yanzhe Cheng, Zitian Chen, Yu Cao, Haiying Zhao, Zhou Tong, Yaning Chang, Yingjun Zhou

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Abstract

Background: Diet plays a significant role in mitigating inflammation, with lettuce and its polyphenols showing potential for improving inflammatory conditions. However, research on the material basis and mechanisms of action remains unclear.

Results: This study investigated the primary components and anti-inflammatory effects of the new lettuce cultivar Binfen-1, which is rich in polyphenols and may offer enhanced health benefits. There were 65 metabolites with significant changes in Binfen-1 by metabolomics, comprising mainly polyphenols. Meanwhile, the high-polyphenol lettuce extract (HPLE) from Binfen-1 demonstrated superior antioxidant and anti-inflammatory effects in vitro. Moreover, HPLE showed a significant improvement in mice with dextran sodium sulfate-induced ulcerative colitis. By integrating network pharmacology with high-performance liquid chromatographic quantitative results, isoquercitrin and chlorogenic acid were identified as the core active substances in HPLE, and the PI3K-Akt, MAPK, and JAK-STAT pathways were the key anti-inflammatory pathways for HPLE, supported by mRNA validation and molecular docking predictions.

Conclusion: The core anti-inflammatory substances in HPLE were isoquercitrin and chlorogenic acid, with the key anti-inflammatory pathways being the PI3K-Akt, MAPK, and JAK-STAT pathways. These findings substantially propel the understanding of the therapeutic potential of lettuce in the treatment of inflammatory diseases. © 2025 Society of Chemical Industry.

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代谢组学和网络药理学揭示了高多酚莴苣新品种抗炎核心物质及其作用机制。

背景：饮食在减轻炎症方面起着重要作用，生菜及其多酚显示出改善炎症状况的潜力。然而，对其物质基础和作用机制的研究尚不清楚。结果：本研究研究了莴苣新品种宾芬1号的主要成分及其抗炎作用，该品种富含多酚类物质，可能具有增强的健康益处。通过代谢组学分析，Binfen-1有65种代谢物发生显著变化，主要是多酚类。同时，宾芬-1高多酚莴苣提取物（HPLE）在体外表现出良好的抗氧化和抗炎作用。此外，HPLE在右旋糖酐硫酸钠诱导的溃疡性结肠炎小鼠中表现出显著改善。结合网络药理学和高效液相色谱定量结果，确定异槲皮苷和绿原酸是HPLE的核心活性物质，PI3K-Akt、MAPK和JAK-STAT通路是HPLE的关键抗炎通路，并得到mRNA验证和分子对接预测的支持。结论：HPLE的核心抗炎物质为异槲皮苷和绿原酸，关键抗炎通路为PI3K-Akt、MAPK和JAK-STAT通路。这些发现极大地推动了对生菜治疗炎症性疾病的治疗潜力的理解。©2025化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.