Investigating the separation and purification of flavonoids extracted from foxtail millet, with an in-depth study on its functions in alleviating metabolic syndrome through suppressing lipid absorption.

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-04-26 DOI:10.1002/jsfa.14295

Xiaoqin La, Xiaoting He, Jingyi Liang, Zhaoyan Zhang, Zhuoyu Li

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引用次数: 0

Abstract

Background: Rising living standards alongside high-fat diets (HFDs) have fueled the prevalence of metabolic syndrome (MetS), posing a significant health challenge with limited effective therapies. Foxtail millet is recognized as a health food with rich flavonoids. The objective of this work is to isolate and purify flavonoids from foxtail millet, which have the potential to alleviate MetS and to clarify their mechanism.

Results: Our research combined single-factor experiments and a Box-Behnken design to optimize the extraction processes for millet whole-grain flavonoids (MWGFs) followed by the purification using D101 resin. Components were eluted using varied alcohol concentrations. Additionally, the investigation revealed that MWGF30 notably decreased triglyceride (TG) levels, mitigated lipid uptake in Caco-2 cells induced by free fatty acids, and suppressed lipid absorption in HFD mice while modulating TG, high‑ and low-density lipoprotein cholesterol and total cholesterol levels. Further investigation uncovered that MWGF30 achieved this lipid uptake reduction ability by downregulating the CD36 protein level.

Conclusion: Collectively, MWGF30 demonstrated a potent ability to regulate blood lipids and curb lipid absorption, enhancing our comprehension of millet's benefits and furnishing a scientifically grounded dietary approach for MetS. © 2025 Society of Chemical Industry.

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对谷子黄酮的分离纯化进行研究，深入研究其通过抑制脂质吸收缓解代谢综合征的作用。

背景：生活水平的提高和高脂肪饮食（HFDs）推动了代谢综合征（MetS）的流行，这对有效治疗有限的健康构成了重大挑战。谷子是公认的富含黄酮类化合物的保健食品。本研究旨在从谷子中分离纯化黄酮类化合物，研究其对代谢代谢综合征的缓解作用及机制。结果：本研究结合单因素实验和Box-Behnken设计优化了谷子全谷总黄酮的提取工艺，并采用D101树脂进行纯化。使用不同浓度的酒精洗脱成分。此外，研究发现MWGF30显著降低甘油三酯（TG）水平，减轻游离脂肪酸诱导的ccao -2细胞的脂质摄取，抑制HFD小鼠的脂质吸收，同时调节TG、高、低密度脂蛋白胆固醇和总胆固醇水平。进一步的研究发现，MWGF30通过下调CD36蛋白水平来实现这种脂质摄取减少能力。结论：总的来说，MWGF30显示出调节血脂和抑制脂质吸收的强大能力，增强了我们对小米益处的理解，并为MetS提供了科学依据的饮食方法。©2025化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.