{"title":"Integrated microbiome and metabolome approaches reveal the resistant mechanisms of leaf blight resistant plum cultivar","authors":"Xinyan Zhou, Yufei Wei, Yu Zhu, Jiaoming Li, Runche Zhou, Qingju Xiao, Ruihong Luo, Shangdong Yang","doi":"10.1186/s40538-025-00780-4","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Endophytic microbiome and metabolome are closely related to the plant resistance. Unravelling the compositional features of the endophytic microbiome and metabolome can help to understand plant resistant mechanisms. This study investigates the resistant mechanisms of leaf blight-resistant plum cultivars through comparative analysis of endophytic microbiome and metabolome features between leaf blight resistant (Mihuang Plum, RP) and susceptible (Pearl Plum, SP) cultivars.</p><h3>Results</h3><p>The results showed that higher microbial diversity and richness could be detected in RP cultivar than those of SP cultivar. Meanwhile, the endophytic bacterial genera, such as <i>1174-901-12</i>, <i>Sneathia</i>, <i>Gardnerella</i>, <i>Bacteroides</i>, <i>Prevotella</i>, <i>Fastidiosipila</i>, and the endophytic fungal genera, <i>Paramycosphaerella</i>, <i>Epicoleosporium</i>, <i>Zasmidium</i>, and <i>Zeloasperisporium</i> were enriched in the RP cultivar. Moreover, in comparison with SP cultivar, Flavonoid biosynthesis, Isoflavonoid biosynthesis, Phenylalanine metabolism, Phenylpropanoid biosynthesis, and Nucleotide metabolism showed high expression levels in RP cultivar, and the contents of (-)-Naringenin, 4-Coumaric acid, Epicatechin, Genistein, M-Coumaric acid, Dihydrokaempferol, and 4-Hydroxycinnamic acid were also significantly higher in RP cultivar.</p><h3>Conclusions</h3><p>Our findings had revealed that significant differences of endophytic microbiome and metabolome features could be found between leaf blight resistant (Mihuang Plum, RP) and susceptible (Pearl Plum, SP) cultivars. Meanwhile, positive correlations between potential functional microorganisms, metabolites and leaf blight resistant plum cultivar also had been identified. In future research, a deeper exploration of these microbial communities and metabolites is warranted to fully understand their functional roles in the resistance mechanisms. Identifying the specific microbial taxa and metabolites that contribute to leaf blight resistance could provide valuable insights into their potential applications in biological control. By harnessing these natural microbial and metabolic resources, it may be possible to develop sustainable, eco-friendly strategies for managing leaf blight, ultimately reducing the dependency on chemical pesticides.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":512,"journal":{"name":"Chemical and Biological Technologies in Agriculture","volume":"12 1","pages":""},"PeriodicalIF":5.2000,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-025-00780-4","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical and Biological Technologies in Agriculture","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1186/s40538-025-00780-4","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Background
Endophytic microbiome and metabolome are closely related to the plant resistance. Unravelling the compositional features of the endophytic microbiome and metabolome can help to understand plant resistant mechanisms. This study investigates the resistant mechanisms of leaf blight-resistant plum cultivars through comparative analysis of endophytic microbiome and metabolome features between leaf blight resistant (Mihuang Plum, RP) and susceptible (Pearl Plum, SP) cultivars.
Results
The results showed that higher microbial diversity and richness could be detected in RP cultivar than those of SP cultivar. Meanwhile, the endophytic bacterial genera, such as 1174-901-12, Sneathia, Gardnerella, Bacteroides, Prevotella, Fastidiosipila, and the endophytic fungal genera, Paramycosphaerella, Epicoleosporium, Zasmidium, and Zeloasperisporium were enriched in the RP cultivar. Moreover, in comparison with SP cultivar, Flavonoid biosynthesis, Isoflavonoid biosynthesis, Phenylalanine metabolism, Phenylpropanoid biosynthesis, and Nucleotide metabolism showed high expression levels in RP cultivar, and the contents of (-)-Naringenin, 4-Coumaric acid, Epicatechin, Genistein, M-Coumaric acid, Dihydrokaempferol, and 4-Hydroxycinnamic acid were also significantly higher in RP cultivar.
Conclusions
Our findings had revealed that significant differences of endophytic microbiome and metabolome features could be found between leaf blight resistant (Mihuang Plum, RP) and susceptible (Pearl Plum, SP) cultivars. Meanwhile, positive correlations between potential functional microorganisms, metabolites and leaf blight resistant plum cultivar also had been identified. In future research, a deeper exploration of these microbial communities and metabolites is warranted to fully understand their functional roles in the resistance mechanisms. Identifying the specific microbial taxa and metabolites that contribute to leaf blight resistance could provide valuable insights into their potential applications in biological control. By harnessing these natural microbial and metabolic resources, it may be possible to develop sustainable, eco-friendly strategies for managing leaf blight, ultimately reducing the dependency on chemical pesticides.
期刊介绍：
Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture.
This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population.
Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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