Computational chemistry-based hapten design and antibody production for the immunochromatographic assay of maleic hydrazide in food and environmental samples

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-05-14 DOI:10.1016/j.foodchem.2025.144723

Feng He, Lingling Guo, Liqiang Liu, Xinxin Xu, Chuanlai Xu, Hua Kuang, Maozhong Sun

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Abstract

Maleic hydrazide (MH), a selective herbicide and plant growth inhibitor, poses risks to ecological balance and human health due to its widespread use. In this study, we developed a simple, reliable, and rapid detection method for MH. Four haptens were analyzed using computational chemistry simulations, and a matrix effect-enhanced screening method was used to obtain the anti-MH monoclonal antibody, with a half-maximal inhibitory concentration (IC₅₀) of 0.157 μg/mL. An immunochromatographic assay was developed for detecting MH in lake water, soil, onion, garlic, potato, and tobacco samples. The IC₅₀ values ranged from 0.590 to 9.419 mg/kg, with a detection limit of 0.047–1.614 mg/kg. Spiked recovery and real sample analyses demonstrated results consistent with instrumental methods, achieving recoveries of 89.2 %–111.3 % and CV < 7 %. These findings confirm the method's suitability for detecting MH in food and environmental samples, fulfilling the requirements for on-site rapid detection in resource-limited settings.

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食品和环境样品中马来酰肼免疫层析分析中基于计算化学的半抗原设计和抗体生产

马来酰肼（MH）是一种选择性除草剂和植物生长抑制剂，其广泛使用给生态平衡和人类健康带来了风险。本研究建立了一种简便、可靠、快速的MH检测方法，通过计算化学模拟对4种半抗原进行分析，采用基质效应增强筛选法获得抗MH单克隆抗体，其半最大抑制浓度（IC50）为0.157 μg/mL。建立了一种检测湖泊水、土壤、洋葱、大蒜、马铃薯和烟草样品中MH的免疫层析方法。IC50范围为0.590 ~ 9.419 mg/kg，检出限为0.047 ~ 1.614 mg/kg。加标回收率和实际样品分析结果与仪器方法一致，回收率为89.2% % -111.3 %，CV为 <； 7 %。这些结果证实了该方法对食品和环境样品中MH检测的适用性，满足了资源有限环境下现场快速检测的要求。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.