Fitness and Resistance Mechanism of Fusarium graminearum from Fields to Demethylation Inhibitor (DMI) Fungicides in China.

IF 5.7 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-05-14 DOI:10.1021/acs.jafc.5c02836

Zhili Yuan,Huina Ma,Yueming Jin,Zelong Peng,Chenguang Wang,Yiping Hou

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Abstract

Tebuconazole and prothioconazole are triazole sterol demethylation inhibitor (DMI) fungicides widely used for controlling Fusarium head blight (FHB) in the world. In 2023, 3714 Fusarium graminearum isolates were collected from fields of 8 provinces in China, among which 116 (3.12%) isolates and 82 (2.21%) isolates showed low resistance to tebuconazole (Teb-R, resistance factor [RF] = 2.65-7.65) and prothioconazole (Pro-R, RF = 3.85-6.04), respectively. Importantly, 11 isolates (0.30%) showed dual resistance (Teb-/Pro-R). Teb-R, Pro-R, and Teb-/Pro-R isolates displayed fitness costs in vegetative growth, asexual and sexual reproduction, or pathogenicity. There was partial cross-resistance among the DMI fungicides. No mutations were found in CYP51s of Teb-R, Pro-R, and Teb-/Pro-R isolates. Under tebuconazole treatment, the expression of CYP51B in Teb-R isolates and CYP51A/B and ABC4 in Teb-/Pro-R isolates was significantly higher than in sensitive isolates. Under prothioconazole treatment, the expression of CYP51A in Pro-R isolates and CYP51A/B in Teb-/Pro-R isolates was significantly higher than that in sensitive isolates.

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中国田间禾谷镰刀菌对去甲基化抑制剂（DMI）杀菌剂的适应性及抗性机制

Tebuconazole和prothioconazole是三唑类甾醇去甲基化抑制剂（DMI）类杀菌剂，目前国际上广泛用于防治赤霉病（Fusarium head疫病）。2013年在全国8个省的田间共分离到3714株稻瘟病镰刀菌，其中对戊康唑（Teb-R，抗性因子[RF] = 2.65 ~ 7.65）和原硫康唑（Pro-R, RF = 3.85 ~ 6.04）低抗性116株（3.12%）和82株（2.21%）。重要的是，11株（0.30%）菌株显示双重耐药（Teb-/Pro-R）。Teb- r、Pro-R和Teb-/Pro-R分离株在营养生长、无性繁殖和有性繁殖或致病性方面显示出适应性成本。DMI杀菌剂之间存在部分交叉抗性。Teb- r、Pro-R和Teb-/Pro-R分离株的CYP51s未发现突变。Teb- r分离株中CYP51B和Teb-/Pro-R分离株中CYP51A/B和ABC4的表达量显著高于敏感株。在原硫康唑处理下，Pro-R分离株CYP51A和Teb-/Pro-R分离株CYP51A/B的表达均显著高于敏感株。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.