Proteomics and physiologic analysis reveal different response strategies to cadmium stress in Lentinula edodes

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-05-12 DOI:10.1016/j.foodchem.2025.144739

Yansha Wu , Dandan Zhai , Ning Jiang , Qiaozhen Li , Meiyan Zhang , Chunyan Song , Haoran Dong , Xiaodong Shang , Hao Yu , Hailong Yu

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Abstract

Lentinula edodes (L. edodes) is the second most widely cultivated edible mushroom worldwide. However, it has the ability to accumulate cadmium (Cd), which poses significant health risks. Despite its significance, the protein-level response mechanisms to Cd stress remain insufficiently understood. This study aims to investigate the differential responses of the low-Cd-accumulating strain Le4606 and the high-Cd-accumulating strain Le4625 under Cd stress by biochemical and proteomic methodologies. The results indicate that Le4625 exhibits enhanced Cd absorption, proline accumulation, and vacuolar sequestration for detoxification, with ZRC1 detected exclusively at 7 h. Conversely, Le4606 demonstrates proficiency in glutathione-mediated detoxification, thioredoxin antioxidant activity, tricarboxylic acid cycle activity, autophagy, and Cd extrusion. Overall, vacuolar sequestration and glutathione-mediated detoxification are important for the differences in Cd accumulation. The distinct response strategies offer valuable insights into the underlying mechanisms of Cd accumulation. This research establishes a theoretical foundation for the breeding of low-Cd-accumulating cultivars, benefiting human health.

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蛋白质组学和生理分析揭示了香菇对镉胁迫的不同响应策略

香菇（Lentinula edodes）是世界上种植面积第二大的食用菌。然而，它具有积累镉（Cd）的能力，这对健康构成重大威胁。尽管具有重要意义，但蛋白质水平对Cd胁迫的反应机制仍不充分了解。本研究旨在通过生物化学和蛋白质组学方法研究低Cd积累菌株Le4606和高Cd积累菌株Le4625在Cd胁迫下的差异反应。结果表明，Le4625表现出增强的Cd吸收、脯氨酸积累和空泡固存以解毒，而ZRC1仅在7 h时检测到。相反，Le4606表现出对谷胱甘肽介导的解毒、硫氧还蛋白抗氧化活性、三羧酸循环活性、自噬和Cd挤压的精通。总的来说，空泡隔离和谷胱甘肽介导的解毒对镉积累的差异很重要。不同的反应策略为Cd积累的潜在机制提供了有价值的见解。本研究为选育低cd积累品种，造福人类健康奠定了理论基础。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.