Detection of some secondary metabolites of Beauveria bassiana and the potential effects on Spodoptera littoralis

IF 5.2 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Chemical and Biological Technologies in Agriculture Pub Date : 2025-05-12 DOI:10.1186/s40538-025-00774-2

Wessam Houssiny Bakr, Mohemed Farouk Ghaly, Wafaa Ghatwary Tantawy, Samah Nour El-Shafeiy

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Abstract

Background

Beauveria bassiana is one of the most renowned entomopathogenic fungi and has recently been recognized as a potential biopesticide for a wide range of insect pests. The secondary metabolites of B. bassiana are believed to contribute to its pesticidal activity. Meanwhile, the Egyptian cotton leafworm, Spodoptera littoralis, is a significant pest that causes considerable damage to various key crops.

Results

In this study, secondary metabolites were extracted using ethyl acetate from both the culture medium filtrate and the mycelia separately. Subsequently, the extracts were separated via thin-layer chromatography into six fractions from the medium filtrate (F0:F5) and ten fractions from the mycelia (M1:M10). All fractions were assessed for their insecticidal effects on second instar larvae of S. littoralis. The treatment of F0 resulted in the highest larval mortality rate of 61.11%, followed by M3 with a mortality rate of 45.19%. M2 and M8 had similar effects, each resulting in a mortality rate of 40.00%. Furthermore, scanning electron microscopy revealed cuticle disorders and deformations in larvae treated with all treatments. Fractions exhibited the highest mortality rates in S. littoralis larvae, and the total injuries were analyzed using GC/MS, which revealed the presence of eight bioactive compounds. Notably, this study represents the first detection of 2,4-di-tert-butylphenol in the mycelia of B. bassiana, a compound known for its high toxicity. In addition, other compounds contributed to the observed experimental results.

Conclusion

These findings indicate that B. bassiana could serve as a sustainable source of bioactive metabolites with significant insecticidal effects on S. littoralis. This suggests a strong potential for improving integrated pest management (IPM), particularly when large-scale production is implemented.

Graphical abstract

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白僵菌次生代谢产物的检测及其对滨海夜蛾的潜在影响

白僵菌（beauveria bassiana）是最著名的昆虫病原真菌之一，近年来被认为是一种潜在的生物农药。球孢白僵菌的次生代谢物被认为有助于其杀虫活性。与此同时，埃及棉叶虫（Spodoptera littoralis）是一种严重危害重要作物的害虫。结果用乙酸乙酯分别从培养基滤液和菌丝中提取次生代谢物。随后，通过薄层色谱分离培养基滤液中的6个组分（F0:F5）和菌丝体中的10个组分（M1:M10）。评价了各组分对滨夜蛾二龄幼虫的杀虫效果。F0处理的幼虫死亡率最高，为61.11%，M3处理次之，为45.19%。M2和M8的效果相似，死亡率均为40.00%。此外，扫描电镜显示，所有处理的幼虫角质层紊乱和变形。结果表明，各馏分对滨海夜蛾幼虫的致死率最高，GC/MS分析结果表明，其中含有8种生物活性化合物。值得注意的是，这项研究首次在球孢白杆菌的菌丝中检测到2,4-二叔丁基酚，这种化合物以其高毒性而闻名。此外，其他化合物对观察到的实验结果也有贡献。结论球孢白僵菌具有良好的杀虫效果，是一种可持续的生物活性代谢物来源。这表明，特别是在实施大规模生产的情况下，改进病虫害综合管理（IPM）的潜力很大。图形抽象

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemical and Biological Technologies in Agriculture Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

6.80

自引率

3.00%

发文量

审稿时长

15 weeks

期刊介绍： Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture. This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population. Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.