Physiological, biochemical and histological effects of abscisic acid-synthesized silver and copper oxide nanoparticles on the potato tuber moth, Phthorimaea operculella

IF 5.2 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Chemical and Biological Technologies in Agriculture Pub Date : 2025-05-10 DOI:10.1186/s40538-025-00782-2

Amr S. Abou El-Ela, Chao Zhang, Asim Munawar, Xuan Chen, Yixin Zhang, Eric Siaw Ntiri, Modhi O. Alotaibi, Amr Elkelish, Suhailah S. Aljameel, Wenwu Zhou, Zeng-Rong Zhu

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引用次数: 0

Abstract

Background

The potato tuber moth (PTM), Phthorimaea operculella, poses a significant threat to potato cultivation in tropical and subtropical regions. Chemical control, though widely used, poses risks to human and environmental health, necessitating safer alternatives. This study investigated eco-friendly alternatives by synthesizing silver (AgNPs) and copper oxide nanoparticles (CuONPs) using abscisic acid (ABA).

Results

Characterization through UV–vis spectroscopy, scanning electron microscopy, transmission electron microscopy, energy dispersive X-ray, X-ray diffraction, Fourier transform infrared spectroscopy, and zeta potential analysis confirmed the successful synthesis of AgNPs with a uniform size of 35.8 nm and a plasmon resonance at 430 nm. CuONPs displayed a porous, rugby sheet-like structure, with a thickness of 68.5 nm and a 290 nm absorption peak. The effectiveness of the nanoparticles against PTM larvae was assessed through larval spraying and leaf-dipping. Larval spraying outperformed leaf-dipping, with median lethal concentration (LC₅₀) values of 670 mg/L for AgNPs and 1320 mg/L for CuONPs. AgNPs and CuONPs significantly altered digestive enzyme activities in treated PTM larvae, reducing α-amylase, β-glucosidase, lipase, protease, and trypsin, and increasing acid phosphatase activity. Histological studies revealed damage to the larvae's midgut epithelial layer and peritrophic membrane, resulting in nuclei dispersion.

Conclusions

Our study shows a cost-effective, minimal-impact strategy for synthesizing AgNPs and CuONPs, which can serve as a potential approach for managing PTM.

Graphical abstract

查看原文本刊更多论文

脱落酸合成的氧化银和氧化铜纳米粒子对马铃薯块茎蛾生理、生化和组织学的影响

马铃薯块茎蛾（Phthorimaea operculella）对热带和亚热带地区的马铃薯种植构成严重威胁。化学控制虽然广泛使用，但对人类和环境健康构成风险，因此需要更安全的替代品。本研究利用脱落酸（ABA）合成银纳米粒子（AgNPs）和氧化铜纳米粒子（CuONPs）。结果通过紫外-可见光谱、扫描电镜、透射电镜、能量色散x射线、x射线衍射、傅里叶变换红外光谱和zeta电位分析等手段进行表征，证实成功合成了尺寸为35.8 nm、在430 nm处有等离子体共振的AgNPs。CuONPs具有多孔的橄榄球片状结构，厚度为68.5 nm，吸收峰为290 nm。采用幼虫喷雾和叶片浸渍两种方法评价纳米颗粒对PTM幼虫的防治效果。喷幼虫对AgNPs的中位致死浓度（LC50）为670 mg/L，对CuONPs的中位致死浓度（LC50）为1320 mg/L。AgNPs和CuONPs显著改变了PTM幼虫的消化酶活性，降低了α-淀粉酶、β-葡萄糖苷酶、脂肪酶、蛋白酶和胰蛋白酶的活性，提高了酸性磷酸酶的活性。组织学研究表明，幼虫的中肠上皮和营养周膜受到损伤，导致细胞核分散。结论我们的研究为合成AgNPs和CuONPs提供了一种成本效益高、影响最小的策略，可作为治疗PTM的潜在方法。图形抽象

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来源期刊

Chemical and Biological Technologies in Agriculture Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

6.80

自引率

3.00%

发文量

审稿时长

15 weeks

期刊介绍： Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture. This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population. Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.