Sedentary chromosomal integrons as biobanks of bacterial antiphage defense systems

IF 45.8 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Pub Date : 2025-05-08

Baptiste Darracq, Eloi Littner, Manon Brunie, Julia Bos, Pierre Alexandre Kaminski, Florence Depardieu, Weronika Slesak, Kevin Debatisse, Marie Touchon, Aude Bernheim, David Bikard, Frédérique Le Roux, Didier Mazel, Eduardo P. C. Rocha, Céline Loot

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Abstract

Integrons are genetic systems that drive bacterial adaptation by acquiring, expressing, and shuffling gene cassettes. While mobile integrons are well known for spreading antibiotic resistance genes, the functions of the hundreds of cassettes carried by sedentary integrons remain largely unexplored. We show that many of these cassettes encode small variants of known antiphage systems that favor their inclusion in the integron. We also demonstrate that nearly 10% of the integron cassettes in the pandemic Vibrio cholerae strain encode novel antiphage functions. Most of these novel systems have little or no similarity to previously known ones, with several providing defense through cell lysis or growth arrest. Our work highlights the stabilization and prevalence of small antiphage systems within integrons, making them an untapped biobank of defense mechanisms.

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静止染色体整合子作为细菌抗噬菌体防御系统的生物库

整合子是通过获取、表达和重组基因磁带来驱动细菌适应的遗传系统。虽然众所周知，移动整合子会传播抗生素抗性基因，但久坐整合子携带的数百个“磁带”的功能在很大程度上仍未被探索。我们表明，许多这些盒式编码的小变种已知的抗菌体系统，有利于其包含在整合子。我们还证明，在大流行霍乱弧菌菌株中，近10%的整合子盒式编码新的抗噬菌体功能。大多数这些新系统与以前已知的系统很少或没有相似之处，其中一些通过细胞裂解或生长抑制提供防御。我们的工作强调了整合子内小噬菌体系统的稳定性和普遍性，使它们成为尚未开发的防御机制生物库。

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来源期刊

Science 综合性期刊-综合性期刊

CiteScore

61.10

自引率

0.90%

发文量

审稿时长

2.1 months

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